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Effect of Bulhwangeumjeonggi-san on Cytokine Levels of Mouse Th1/Th2 Cells and Anti-allergic Activity in Ovalbumin-sensitized Allergic Inflammation Model  

Lim, Kang-Min (Department of oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University)
Kang, Hee (Department of oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University)
Park, Sung-Min (Department of oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University)
Shim, Bum-Sang (Department of oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University)
Kim, Sung-Hoon (Department of oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University)
Choi, Seung-Hoon (Department of oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University)
Ahn, Kyoo-Seok (Department of oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.6, 2006 , pp. 1467-1476 More about this Journal
Abstract
This study was to evaluate the effect of Bulhwangeumjeonggi-san (BS) on mouse Th1 and Th2 cells' differentiation and ovalbumin (OVA)-induced allergic inflammation. The proliferation of mouse CD4 T cells and the secretion of Th1/Th2 cytokines under the influence of BS extract were measured as well as the amount ${\beta}$-hexosaminidase in RBL-wH3 cells and the levels of TNF-${\alpha}$ and IL-6 secretion in Raw264.7 cells. BALB/c mice were orally administered with BS extract and simultaneously inoculated with OVA to induce allergic reaction and measure the level of total lgE, OVA-specific lgE and the production of IFN- g, IL-4, IL-5 by the spleen cells. When mouse CD4- T cell were stimulated with anti-CD3 and anti-CD28 for 48 hours in various concentrations of BS extract, it increased proliferation of CD4 cells by 14% in 50 ${\mu}g/m{\ell}$ concentration but it showed an inhibition in higher concentrations. CD4 T cells under Th1/Th2 polarizing conditions for 3 days with BS resulted in mild decrease of IFN- g in Th1 cells and mild increase of IL-4 in Th2 cell at 50 ${\mu}g/m{\ell}$ but the level of IL-4 decreased by 18% at 100 ${\mu}g/m{\ell}$. BS extract had a dose-dependent inhibitory effect on antigen-induced release of ${\beta}$-hexosaminidase in RBL-2H3 cells. Treatment of BS extract on LPS stimulated Raw 264.7 cells showed dose-dependent decrease in TNF-${\alpha}$ production. Oral administration of BS extract on OVA-induced allergic mice showed an inhibitory effect on the levels of total serum lgE and OVA-specific lgE by 50% and 55%, respectively. Culture of spleen cells with OVA resulted in significant increase of IFN- g by 25% and significant decrease of IL-4 and IL-5 by 53%, and 38%, respectively. The results show that BS does not strongly induce mouse T cells to transform into Th1 or Th2 but it has an anti-allergic effect in vitro, and that it also corrects the unbalance between the reactions of Th cells in allergic diseases.
Keywords
Bulhwangeumjeonggi-san; Th1/Th2; IL-4; IFN- g; Allergy;
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