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Purification of Total Ginsesides with Macroporous Resins and Their Biological Activities  

Li, Huayue (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Jin, Haizhu (Department of Biochemistry, Yantai University)
Lee, Dong-Geun (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Lee, Jae-Hwa (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Ha, Bae-Jin (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Ha, Jong-Myung (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.5, 2006 , pp. 1321-1326 More about this Journal
Abstract
Total ginsenosides were purified and their antioxidant, antibacterial and anticancer activities were measured. The crude extracts of ginseng, which were extracted with 75% ethanol by ultrasonification method, were firstly purified on AB-8 macroporous adsorption column to remove water soluble impurities, and decolored on Amberlite IRA 900 Cl anion-exchange column. Then, they were purified on Amberlite XAD16 adsorption column to delete the non-polar impurities. Total ginsenosides contents of the purified extracts were 79.4%, 71.7% and 72.5% in cultured wild ginseng, red ginseng and white ginseng, which were significantly increased than those of crude extracts. All of the three extracts showed concentration-dependant scavenging activities against DPPH radicals, among which white ginseng showed the most powerful activity. Cultured wild ginseng roots showed strongest effect against both B. subtilis PM 125(Gram-positive) and E. coli D31 (Gram-negative) bacteria, while red ginseng and white ginseng only showed the activity against B. subtilis. According to the result of the MTT assay, ail of the three extracts inhibited the growth of U-937 human hohistiocytic lympma cell, which were significantly different (p < 0.05) when compared to the control.
Keywords
ginsenoside; macroporous resin; purification; antioxidant; antibacterial; anticancer;
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