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Mechanisms of Siegesbeckia Glabrescens-induced Smooth Muscle Cell Apoptosis: Role of iNOS and PKC${\alpha}$  

Lee, Seung-Yeul (Department of Physiology, College of Oriental Medicine, Dongguk University)
Jun, Soo-Young (Department of Physiology, College of Oriental Medicine, Dongguk University)
Kim, Jong-Bong (Department of Physiology, College of Oriental Medicine, Dongguk University)
Jang, Hyo-Oil (Department of Physiology, College of Oriental Medicine, Dongguk University)
Kim, Gil-Whon (Department of Physiology, College of Oriental Medicine, Dongguk University)
Shin, Heung-Mook (Department of Physiology, College of Oriental Medicine, Dongguk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.5, 2006 , pp. 1233-1240 More about this Journal
Abstract
We have recently demonstrated that Siegesbeckia glabrescens(SG), a herbal medicine, induces apoptosis via nitric oxide(NO) production in human aortic smooth muscle cells(HASMCS). However, the molecular pathways involved in SG-mediated apoptosis are not fully understand. In the present study, we investigated the cellular mechanisms of SG-induced apoptosis in HASMCS. SG induced NO production through inducible nitric oxide synthase(iNOS) induction. The apoptotic effect of SG was attenuated by L-NNA, a NOS inhibitor. In the presence of L-NNA, the degradation of procaspase-3 by SG was inhibited. SG treatment induced a decrease in Bcl-2 expression but did not affect the expression of Bax. In addition, SG treatment evoked both down-regulation of PKC ${\alpha}$ and inhibition of PKC ${\alpha}$ phosphorylation. These downregulations were reversed by addition of L-NNA. It seems likely to De a downregulation of PKC${\alpha}$ due to long term treatment with PMA. Taken together, these results suggest that apoptotic effects of SG may be due to NO production via iNOS mRNA expression. Furthermore, Bcl-2 and PKC${\alpha}$ downregulation, and caspase-3 activation may be involved in the mechanisms for apoptotic effects by SG.
Keywords
Siegesbeckia glabrescens Makino(SG); Human aortic smooth muscle cell; Apoptosis; iNOS; Bcl-2; PKC${\alpha}$; Caspase-3;
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