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Effects of Traditional Wine by using Mycelium of Phellinus Iinteus on the Expression of Inflammation-Related Proteins in HepG2 Cells  

Lee, Su-Jin (Departments of Anatomy, College of Oriental Medicine, Dong-Eui University)
Han, Min-Ho (Department of Biochemistry, College of Oriental Medicine, Dong-Eui University)
Lee, Yong-Tae (Department of Physiology, College of Oriental Medicine, Dong-Eui University)
Huh, Man-Kyu (Department of Molecular Biology, College of Natural Science, Dong-Eui University)
Chung, Kyung-Tae (Department of Life Science and Biotechnology, College of Natural Science, Dong-Eui University)
Jeong, Young-Kee (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
Choi, Yung-Hyun (Department of Biochemistry, College of Oriental Medicine, Dong-Eui University)
Choi, Byung-Tae (Departments of Anatomy, College of Oriental Medicine, Dong-Eui University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.4, 2006 , pp. 914-918 More about this Journal
Abstract
It was examined that the effect of fermented traditional wine made by using mycelium of Phellinus linteus on the expression of inflammation-related proteins in HepG2 cells. HepG2 cells were incubated with or without ertract of traditional wine (ETMP), then analyzed by microscopic observation, reverse transcription polymerase chain reaction (RT-PCR) and Western blot. The results of RT-PCR and Western blot analyses showed that the level of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2 and tumor necrosis factor $(TNF)-{\alpha}$ was induced by LPS, Dut the treatment of ETMP inhibited the expression of these proteins and its mRNAs. Besides, the results of Western blot analyses showed that the expression of nuclear $factor-{\kappa}Bp65$ and $inhibitory-{\kappa}B{\alpha}$ were also slightly affected by ETMP treatment. These results suggest that ETM P alleviate the expression of inflammation-related protein expressions and thus may be used as a functional alcoholic beverage.
Keywords
Phellinus Iinteus; mycelum; inflammation; iNOS; COX-2; $TNF-{\alpha}$;
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