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In Vitro Antioxidant and Repaired Effects of the Extracts from Pueraria thunbergiana Roots  

Shin, Sun-Woo (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Shin, Joung-Mi (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Yook, Chan-Nam (Department of Food Nutrition, Wonkwang Health Science College)
Na, Young-Soon (Department of Beauty Science, Konyang University)
Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.4, 2006 , pp. 909-913 More about this Journal
Abstract
The antioxidative activity was observed in the ethyl acetate extract $(IC_{50},\;119{\pm}0.16\;{\mu}g/mL)$ from Pueraria thunbergiana. The DPPH radical scavenging effect of this extract was comparable with that of synthetic antioxdant, BHA $(IC_{50},\;88.39{\pm}1.1\;{\mu}g/mL){\times}\;10^{-3}\;{\mu}M$. Pueraria thunbergiana extracts against microorganisms were evaluated in terms of the minimum inhibitory concentrations (MIC). In general, C. albicans was stronger antimicrobial activity than the other microorganisms. in vitro the antitoxic activity of extracts of Pueraria thunbergiana on NIH 373 fibroblasts was evaluated by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) method. Generally, detoxification effect by the extracts of Pueraria thunbergiana increased in proportion to the concentrations. When $10\;{\mu}g/mL$ of the hexane extract of Pueraria thunbergiana was treated it showed the highest antitoxic effects (p<0.01). These results suggest that the crude extract of P. thunbergiana has a potential therapeutic activity.
Keywords
Pueraria thunbergiana; Antimicrobial activity; Cytotoxicity; Minimum inhibitory concentration (MIC);
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