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Apoptosis-inducing Effect of Takrisodokyeum Extract in Androgen Independent Prostate Cancer Cells  

Lee, Hyung-Jae (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Kwon, Kang-Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Shin, Byung-Cheul (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University)
Kim, Eun-Kyung (Department of Biochemistry, College of Medicine, Chonbuk National University Medical School)
Han, Mi-Jeong (Department of Biochemistry, College of Medicine, Chonbuk National University Medical School)
Song, Mi-Young (Department of Biochemistry, College of Medicine, Chonbuk National University Medical School)
Lee, Young-Rae (Department of Biochemistry, College of Medicine, Chonbuk National University Medical School)
Park, Byung-Hyun (Department of Biochemistry, College of Medicine, Chonbuk National University Medical School)
Ryu, Do-Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.4, 2006 , pp. 861-865 More about this Journal
Abstract
Takrisodokyeum (TRSDY) has been known to exert anti-tumoral activity in Korea. However, its molecular mechanism of action is not understood. In this study, we found that TRSDY induced apoptosis in androgen-independent prostate cancer DU145 cells as evidenced by DNA fragmentation and chromatine condensation in hoechst 33342 dye staining. Our data demonstrated that TRSDY-induced apoptotic cell death was accompanied by increases of PTEN and Par-4 in a time-dependent manner Taken together, these results suggest that TRSDY induce PTEN and Par-4 expression, and eventually lead to apoptotic cell death in androgen independent prostate cancer DU145 cells.
Keywords
Takrisodokyeum(TRSDY); Du145 cell; apoptosis; PTEN; Par-4;
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1 龔廷賢. 增補萬病回春. 台北, 大中國圖書公社, pp 176-182, 1959
2 南采祐. 靑囊訣. 서울, 漢城圖書株式會社, p 92, 1949
3 金在百 外. 病態生理學. 서울, 圖書出版 샤론, p 14, 1984
4 金聖培. 大黃牧丹皮湯이 實驗動物의 鎭痛, 消炎, 鎭靜 및 正常體溫에 미치는 影響. 圓光大學校 大學院, 1991
5 Janus, T.J., Kyritsis, A.P., Forman, A.D., Levin, V.A. Biology and treatment of gliomas. Ann Oncol Review, 3(6):423-433,1992   DOI
6 Yoshida, T., Kawano, N. Clinical cure of glioblastoma-two case reports. Neurol Med Chir (Tokyo), 40(4):224-229, 2000   DOI   ScienceOn
7 Madajewicz, S., Chowhan, N. Therapy for patients with high grade astrocytoma using intraarterial chemotherapy and radiation therapy. Cancer, 88(10):2350-2356, 2000   DOI   ScienceOn
8 Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248-254, 1976   DOI   ScienceOn
9 Gurumurthy, S., Vasudevan, K.M., Rangnekar, V.M. Regulation of apoptosis in prostate cancer. Cancer Metastasis Rev, 20(3-4):225-243, 2001   DOI   ScienceOn
10 Greenlee, R.T., et al. Cancer statistics, 2001. CA Cancer J Clin, 51(1):15-36, 2001   DOI   ScienceOn
11 吉仁浩. 榮衛返魂湯이 實驗動物의 鎭痛, 消炎, 解熱 및 筋 弛緩에 미치는 影響. 圓光大學校 大學院, 1990
12 金定濟 外. 東醫臨床要鑑. 서울, 書苑堂, p 218, 314, 1977
13 康命吉. 濟衆新篇. 서울, 通文舘, p 108, 1968
14 Lu-Yao, G.L., et al. An assessment of radical prostatectomy. Time trends, geographic variation, and outcomes. The Prostate Patient Outcomes Research Team. Jama, 269(20):2633-2636, 1993
15 Chakraborty, M., et al. Par-4 drives trafficking and activation of Fas and Fasl to induce prostate cancer cell apoptosis and tumor regression. Cancer Res, 61(19):7255-7263, 2001
16 吳克潛. 古今醫方集成. 서울, 翰成社(重刊), p 688, 1980
17 熊純生. 辭海. 臺北, 中華書局, p 90, 1974
18 金定濟. 東醫診療要鑑. 서울, 東洋醫學硏究院, 上 p 618, 626, 下 pp 446-447, 1974
19 陳憲定. 丸散膏丹集成. 中國, 大新書局, p 732, 1967
20 Davies, M.A., et al. Regulation of Akt/PKB activity, cellular growth, and apoptosis in prostate carcinoma cells by MMAC/PTEN. Cancer Res, 59(11):2551-2556, 1999
21 Isaacs, J.T., et al. Androgen regulation of programmed death of normal and malignant prostatic cells. J Androl, 13(6):457-464, 1992
22 Kwon, K.B., et al. Vibrio vulnificus cytolysin induces superoxide anion-initiated apoptotic signaling pathway in human ECV304 cells. J Biol Chem, 276(50):47518-47523, 2001   DOI   ScienceOn
23 東西醫學融合硏究會編. 臨床東西醫學. 서울, 영림사, pp 538- 546, 1997
24 Kwon, K.B., et al. Induction of apoptosis by takrisodokyeum through generation of hydrogen peroxide and activation of caspase-3 in HL-60 cells. Life Sci, 73(15):1895-1906, 2003   DOI   ScienceOn
25 Steck, P.A., et al. Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers. Nat Genet, 15(4):356-362, 1997   DOI   ScienceOn
26 許浚. 東醫寶鑑. 서울, 南山堂, 卷二 p 725, 卷三 p 726, 728, 733, 734, 741, 745, 1986
27 蔡炳允. 癰疽에 應用되는 仙方活命飮이 鎭痛, 消炎, 解熱에 미치는 影響. 慶熙大學校 大學院 論文集 三卷, pp 67-90, 1980
28 鄭善充. 內托羌活湯 煎湯液이 實驗動物의 鎭痛, 消炎, 解熱 및 筋弛緩에 미치는 影響. 圓光大學校 大學院, 1990
29 黃德讚. 淸熱消毒飮이 實驗動物의 鎭痛.消炎에 미치는 影響. 圓光大學校 大學院, 1989
30 Widmann, C., Gibson, S. Caspase-dependent cleavage of signaling proteins during apoptosis: a turn-off mechanism for anti-apoptotic signals. J Biol Chem, 273(12):7141-7147, 1998   DOI   ScienceOn
31 Walsh, P.C., Partin, A.W., Epstein, J.I. Cancer control and quality of life following anatomical radical retropubic prostatectomy: results at 10 years. J Urol, 152(5 Pt 2):1831-1836, 1994   DOI
32 杏林書院編. 古今實驗方. 서울, 杏林書院, p 205, 1958
33 朱仁康. 實用外科中藥治療學. 中國, 文光圖書公司, pp 1-3, 15-28, 1957
34 Wyllie, A.H., Kerr, J.F. Cell death: the significance of apoptosis. Int Rev Cytol, 68, 251-306, 1980   DOI