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Effect of Yeongyupaedog-san on Cytokine Levels of Mouse Th1/Th2 Cells and Anti-allergic Activity in Ovalbumin-sensitized Allergic Inflammation Model  

Khwag, Nyo-Gyu (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University)
Kang, Hee (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University)
Myung, Eu-Gene (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University)
Park, Sung-Min (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University)
Shim, Bum-Sang (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University)
Kim, Sung-Hoon (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University)
Choi, Seung-Hoon (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University)
Ahn, Kyoo-Seok (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.4, 2006 , pp. 844-852 More about this Journal
Abstract
This study was to evaluate the effect of Yeongyupaedog-san (YGPDS) on mouse Thl and Th2 cells' differentiation and ovalbumin (OVA)-induced allergic inflammation. The proliferation of mouse CD4 T cells and the secretion of Th1/Th2 cytokines under the influence of YGPDS extract were measured as well as the amount of ${\beta}-hexosaminidase$ in RBL-2H3 cells and the levels of $TNF-{\alpha}$ and 1L-6 secretion in Raw264.7 cells. BALB/c mice were orally administered with YGPDS extract and simultaneously inoculated with OVA to induce allergic reaction and measure the level of total IgE, OVA-specific IgE and the production of IFN- g, IL-4, IL-5 by the spleen cells. When mouse CD4 T cell were stimulated with anti-CD3 and anti-CD28 for 48 hours in various concentrations of YGPDS extract, it increased proliferation of CD4 cells by 11% in $100\;{\mu}g/^{ml}$ concentration but it showed an inhibition by 37% at $200\;{\mu}g/^{ml}$ CD4 T cells under Th1/Th2 polarizing conditions for 3 days with YGPDS resulted in mild decrease of IFN- g in Thl cells and significant decrease of IL-4 in Th2 cells at $500\;{\mu}g/^{ml}\;and\;100\;{\mu}g/^{ml}$ by 18% and 21%, respectively. YGPDS extract had a dose-dependent inhibitory effect on antigen-induced release of ${\beta}-hexosaminidase$ in RBL-2H3 cells. Treatment of YGPDS extract on LPS stimulated Raw 264.7 cells showed dose-dependent decrease in TNF-n production. Oral administration of YGPDS extract on OVA-induced allergic mice showed an inhibitory effect on the levels of total serum IgE and OVA-specific IgE by 25% and 34% , respectively. Culture of spleen cells with OVA resulted in significant increase of IFN- g by 44% and significant decrease of IL-4 and IL-5 by 56%, and 24%, respectively. The results show that YGPDS does not strongly induce mouse T cells to transform into Thl or Th2 but it has an anti-allergic effect in vitro, and that it also corrects the unbalance between the reactions of Th cells in allergic diseases.
Keywords
Yeongyupaedog-san; Th1/Th2; IL-4; IFN- g; allergy;
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