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Regulatory Effects of Gamisamul-tang on Atopic Dermatitis Induced in the NC/Nga Mice  

Yang, Sun-Sim (Department of Pathology, College of Oriental Medicine, Daejeon University)
NamGung, Uk (Department of Neurophysiology, College of Oriental Medicine, Daejeon University)
Kim, Dong-Hee (Department of Pathology, College of Oriental Medicine, Daejeon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.4, 2006 , pp. 1036-1043 More about this Journal
Abstract
The present study was conducted to investigate the effect of Gamisamul-tang (GSMT) on atopic dermatitis (AD). AD was induced in NC/Nga mutant mice by DNCB treatment. GSMT administration reduced levels of skin severity scores. Serum levels of IgE, IgG, IgM, and inflammatory cytokines including IL-4, IL-4 and 1L-13 were significantly decreased by GSMT treatment. Levels of mRNA's encoding IL-4, IL-6, IL-13, $TNF-{\alpha}$, and $interferon-{\gamma}$ in the dermal tissue and draining lymph node (DLN) by real time RT-PCR analysis showed decrease by GSMT testament. Moreover, the number of CD4+ and CD8+ cells was significantly decreased in the spleen and DLN tissues. Histological examination showed that infiltration levels of immune cells in ear, skin, and DLN of AD-induced NC/Nga mice were much improved by GSMT treatment. The present data suggest that GSMT may play an important role in recovering AD symptoms by regulating immune reactivity.
Keywords
Gamisamul-tang; NC/Nga mouse; Atopic dermatitis; RT-PCR; inflammatory cytokine;
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