Browse > Article

Inhibitory effect of Panax notoginseng and emodin on LPS-induced iNOS, COX-2 and prostaglandin E2  

Shin, Jin-Cheul (Department of AM-Pointology, Diagnostics and Cardiovascular Medial Research Center, Dongguk University)
Moon, Jin-Young (Department of AM-Pointology, Diagnostics and Cardiovascular Medial Research Center, Dongguk University)
Park, Won-Hwan (Department of AM-Pointology, Diagnostics and Cardiovascular Medial Research Center, Dongguk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.3, 2006 , pp. 724-729 More about this Journal
Abstract
Many traditional herbal remedies exhibit several beneficial effects including anti-inflammation. The exact mechanism of the a-inflammato action of Panax notoginseng Buck F.H. Chen. however, has not been determined. In the present study, we have isolted the acting compound, emodin, from P. notoginseng and examined the effects of p. notoginseng and emodin on lipopolysaccharide (LPS)-induced nitric oxide (NO) production, and inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) gene expression in RAW264.7 macrophages. The results indicated that p. notoginseng concentration-dependently inhibited LPS-induced NO production. Furthermore, P. notoginseng inhibited the expression of LPS-induced iNOS and COX-2 proteins without an appreciable cytotoxic effect on RAW264.7 cells. Emodin also inhibited LPS-induced iNOS protein as potently as P. notoginseng. This was consistent with the findings that P. notoginseng but not emodin inhibited prostaglandin E2 synthesis induced Dy LPS.
Keywords
iNOS; Cytokines; Cycloxygenase-2(COX-2); lipopolysaccharide(LPS); Prostaglandin E2; emodin; Panax notoginseng;
Citations & Related Records
연도 인용수 순위
  • Reference
1 A. Pedoto, A.K. Tassiopoulos, A. Oler, D.J. McGraw, S.P. Hoffmann, E.M. Camporesi and T.S. Hakim, Treatment of septic shock in rats with nitric oxide synthase inhibitors and inhaled nitric oxide. Crit Care Med 26, 2021-2028, 1998   DOI   ScienceOn
2 C. Cao, K. Matsumura, K. Yamagata and Y. Watanabe, Induction by lipopolysaccharide of cyclooxygenase-2 mRNA in rat brain; its possible role in the febrile response. Brain Res 697, 187-196, 1995   DOI   ScienceOn
3 Wei C.H., Wang. C.F., Chang, L.Y., Tu, Y.C., Yao Hsueh Tung Dao 15, 43-44, 1980
4 J.K. Lin, Y.C. Liang and S.Y. Lin-Shiau. Cancer chemoprevention by tea polyphenols through mitotic signal transduction blockade. Biochem. Pharmacol. 58, 911-915, 1998   DOI   ScienceOn
5 H. Kim, H.S. Lee, K.T. Chang, T.H. Ko, K.J. Baek and N.S. Kwon, Chloromethyl ketones block induction of nitric oxide synthase in murine macrophages by preventing activation of nuclear factor-B. J Immunol 154, 4741-4748, 1995
6 N. Quan, M. Whiteside and M. Herkenham, Cyclooxygenase 2 mRNA expression in rat brain after peripheral injection of lipopolysaccharide. Brain Res 802, 189-197, 1998   DOI
7 Yoshikawa, M.; Morikawa, T.; Yashiro, K.; Murakami, T.; Matsuda, H. Chem. Pharm. Bull. 49, 1452-1456, 2001   DOI   ScienceOn
8 B. Tunctan, O. Uludag, S. Altug and N. Abacioglu, Effects of nitric oxide synthase inhibition in lipopolysaccharide-induced sepsis in mice. Pharmacol Res 38, 405-411, 1998   DOI   ScienceOn
9 K. Aoki, H. Maruta, F. Uchiumi, T. Hatano, T. Yoshida and S. Tanuma, A macrocircular ellagitannin, oenothein B, suppresses mouse mammary tumor gene expression via inhibition of poly(ADP-ribose) glycohydrolase. Biochem Biophys Res Commun 210, 329-337, 1995   DOI   ScienceOn
10 U.K. MeBmer, E.G. Lapetina and B. Brune, Nitric oxide-induced apoptosis in RAW 264.7 macrophages is antagonized by protein kinase C- and protein kinase A-activating compounds. Mol Pharmacol 47, 757-765, 1995.
11 M. Ueno and T.J.-F. Lee, Endotoxin decreases the contractile responses of the porcine basilar artery to vasoactive substances. J Cereb Blood Flow Metab 13, 712-71, 1993   DOI   ScienceOn
12 Wu, M.Z. Yunnan Chih Wu Ten Chiu 1, 119-124, 1979
13 C. Cao, K. Matsumura, K. Yamagata and Y. Watanabe, Induction by lipopolysaccharide of cyclooxygenase-2 mRNA in rat brain; its possible role in the febrile response. Brain Res 697, 187-196, 1995   DOI   ScienceOn
14 Li, X.C., Chinese herbal study on anti-ischemia-reperfusion-injury. Chinese Journal of Hospital Pharmacy 154, 1-4, 1998
15 D.L. Duval, D.R. Miller, J. Collier and R.E. Billings, Characterization of hepatic nitric oxide synthase: Identification as the cytokine-inducible form primarily regulated by oxidants. Mol Pharmacol 50, 277-284, 1996
16 S.H. Lee, E. Soyoola, P. Chanmugam, S. Hart, W. Sun, H. Zhong, S. Liou, D. Simmons and D. Hwang, Selective expression of mitogen-inducible cyclooxygenase in macrophages stimulated with lipopolysaccharide. J Biol Chem 267, 25934-2593, 1992
17 T. Yuan, H.T. Vogel, C. Sutherland and M.P. Walsh, Characterization of the $Ca^{2+}$-dependent and -independent interactions between calmodulin and its binding domain of inducible nitric oxide synthase. FEBS Lett 431, 210-214, 1998   DOI   ScienceOn
18 Namba, T., Matsushige, K., Morita, T., Tanaka, O. Chem. Pharm. Bull. 34, 736-738, 1986
19 Yamaguchi, H., Kasai, R., Matsuura, H., Tanaka, O., Fuwa, T. Chem. Pherm. Bull. 36, 3468-3473, 1988   DOI   ScienceOn
20 M.A. Marletta, Mammalian synthesis of nitrite, nitrate, nitric oxide and N-nitrosating agents. Chem Res Toxicol 1, 249-257, 1988   DOI   ScienceOn
21 Zhou, J., Wu, M.Z., Taniyasu, S., Besso, H., Tanaka, O., Saruwatari, Y., Fuwa, T. Chem. Pharm. Bull. 29, 2844-2850, 1981   DOI
22 M.A. Marletta, P.S. Yoon, R. Iyengar, C.D. Leaf and J.S. Wishnok, Macrophage oxidation of -arginine to nitrite and nitrate: Nitric oxide is an intermediate. Biochemistry 27, 8706-8711, 1988   DOI   ScienceOn
23 E. Haslam, T.H. Lelly, C. Ya, R. Martin and D. Magnolato, Traditional herbal medicine. The role of plant polyphenols. Planta Med 55, 1-8, 1989   DOI   ScienceOn
24 Taniyasu, S., Tanaka, O., Yang, T.-R., Zhou, J. Planta Med. 44, 124-125, 1982   DOI
25 Matsuura, H., Kasai, R., Tanaka, O., Saruwatari, Y., Fuwa, T., Zhou, J., Chem. Pharm. Bull. 31, 2281-228, 1983   DOI
26 Yang, T.-R., Kasai, R., Zhou, J., Tanaka, O., Phytochemistry 22, 1473-1478, 1983   DOI   ScienceOn
27 R.H. Liu and J.H. Hotchkiss, Potential genotoxicity of chronically elevated nitric oxide: A review. Mutat Res 339, 73-89, 1995   DOI   ScienceOn
28 K. Miyamoto, M. Nomura, M. Sasakura, E. Matsui, R. Koshiura, T. Murayama, T. Furukawa, T. Hatano, T. Yoshida and T. Okuda, Anti-tumor activity of oenothein B, a unique macrocyclic ellagitannin. Jpn J Cancer Res 84, 99-103, 1993   DOI
29 H.C. Huang, J.H. Chang, S.F. Tung, R.T. Wu, M.L. Foegh and S.H. Chu, Immunosuppressive effect of emodin, a free radical generator. Eur J Pharmacol 211, 359-364, 1992   DOI   ScienceOn
30 Sanada, S. Shoji, J. Shoyakugaku Zasshi 32, 96-99, 1978
31 S.H. Lee, E. Soyoola, P. Chanmugam, S. Hart, W. Sun, H. Zhong, S. Liou, D. Simmons and D. Hwang, Selective expression of mitogen-inducible cyclooxygenase in macrophages stimulated with lipopolysaccharide. J Biol Chem 267, 25934-25938, 1992
32 K. Yasukawa, M. Takido, M. Takeuchi, Y. Sato, K. Nitta and S. Nakagawa, Inhibitory effects of flavonol glycosides on 12-O-tetradecanoylphorbol-13-acetate-induced tumor promotion. Chem Biol Bull 38, 774-776, 1990
33 S. Moncada, R.M.J. Palmer and D.A. Higgs, Nitric oxide: Physiology, pathophysiology, and pharmacology. Pharmacol Rev 43, 109-142, 1992
34 M.A. Marletta, Nitric oxide synthase structure and mechanism. J Biol Chem 268, 2231-12234, 1993
35 C. Nathan, Nitric oxide as a secretory product of mammalian cells. FASEB J 6, 3051-3064, 1992   DOI
36 J.M. Muller, H.W. Loms Ziegler-Heitbrock and P.A. Baeuerle, Nuclear factor kappa B, a mediator of lipopolysaccharide effects. Immunobiology 187, 233-256, 1993   DOI
37 N. Quan, M. Whiteside and M. Herkenham, Cyclooxygenase 2 mRNA expression in rat brain after peripheral injection of lipopolysaccharide. Brain Res 802, 189-197, 1998   DOI
38 F. Yang, W.J. de Villiers, C.J. McClain and G.W. Varilek. Green tea polyphenols block endotoxin-induced tumor necrosis factor-production and lethality in a murine model. J. Nutr. 128, 2334-2340, 1998   DOI
39 Zhang, G.Q., Ye, R.G., Kong, Q.Y., Yang, N.S., Zhang, J.L., Guan, W.M. and Chen, Y.X., Panax notoginseng saponins induced of human renal interstitial fibroblasts and its mechanisms. Chinese Journal of Nephrology 14(2):93-95, 1998