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Relationship of Inhibitory Effects of Dichroa febrifuga and $IKK{\gamma}$ on the Activation of $NF-{\kappa}B$  

Choi, Byung-Tae (Department of Anatomy, College of Oriental Medicine, Dongeui University)
Lee, Yong-Tae (Department of Physiology, College of Oriental Medicine, Dongeui University)
Hwang, Jang-Sun (Department of Microbiology, College of Natural Sciences, Changwon National University)
Moon, Hae-In (Department of Microbiology, College of Natural Sciences, Changwon National University)
Lee, Kyung-Soo (Department of Microbiology, College of Natural Sciences, Changwon National University)
An, Won-Gun (Department of Nanomedical engineering, College of Nanobiotechnology, Pusan National University)
Kim, Dong-Wan (Department of Microbiology, College of Natural Sciences, Changwon National University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.3, 2006 , pp. 651-656 More about this Journal
Abstract
Activation of $NF-{\kappa}B$ is known to be a trigger of various cellular disorders including inflammatory and autoimmune diseases such as rheumatoid arthritis. Numerous approaches are ongoing within laboratories to identify potential therapeutic agents which inhibit the $NF-{\kappa}B$ activation. In this study, we have tested the inhibitory effects of five traditional medicines on the activation of $NF-{\kappa}B$ by NIK. Among three medicines which exhibited inhibitory effect on the expression of $NF-{\kappa}B$ repoter plasmid, we investigated further the inhibitory mechanism of Dichroa febrifuga in connection with IKKY activity. Wild type $IKK{\gamma}$ inhibited the $NF-{\kappa}B$ activation by NIK but the C-terminal deletion mutant of IKKY did not show the inhibitory effect, indicating that the C-terminal leucine zipper domain of $NF-{\kappa}B$ is important for the inhibition of $NF-{\kappa}B$ activation. The water extract of Dichroa febrifuga(DFE) also strongly inhibited the $NF-{\kappa}B$ activation by NIK. The inhibitory activity of DFE appeared to be independent of the expression of $IKK{\gamma}$, suggesting that the pathways of inhibition by Dichroa febrifuga and $IKK{\gamma}$ are different. Our results suggest that Dichroa febrifuga can be used as a medicine for inhibition of the $NF-{\kappa}B$ activation in a wide range of cells without relation to the expression of $IKK{\gamma}$.
Keywords
Dichroa febrifuga; $NF-{\kappa}B$ activation; $IKK{\gamma}$; NIK;
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