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Effects of the Combined-administration of Ginseng Radix Rubra and Vitis Fructus on Immune Response  

Park, Hun (College of Pharmacy, Woosuk University)
Lee, Kyung-A (College of Pharmacy, Woosuk University)
Jeon, Yong-Keun (College of Pharmacy, Woosuk University)
Leem, Jae-Yoon (College of Pharmacy, Woosuk University)
Shin, Tae-Yong (College of Pharmacy, Woosuk University)
So, June-No (College of Health and Welfare, Woosuk University)
Ahn, Mun-Saeng (Institute of Korea Medicated-Diets)
Kwon, Jin (Korea National College of Rehabilitation & Welfare)
Eun, Jae-Soon (College of Pharmacy, Woosuk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.2, 2006 , pp. 420-427 More about this Journal
Abstract
Immunological activities of the combined-administration of Ginseng Radix Rubra and Vitis Fructus were examined in C57BL/6 mice. Ginseng Radix Rubra and Vitis Fructus were extracted with distilled water or 40% ethyl alcohol. Ginseng Radix Rubra water extracts (GW), the mixture (1:1) of Ginseng Radix Rubra and Vitis Fructus water extracts [GVW(1:1)], the mixture (1:3) of Ginseng Radix Rubra and Vitis Fructus water extracts [GVW(1:3)], 40% ethyl alcohol extracts of Ginseng Radix Rubra (GE), the mixture (1:1) of Ginseng Radix Rubra and Vitis Fructus 40% ethyl alcohol extracts [GVE(1:1)] and the mixture (1:3) of Ginseng Radix Rubra and Vitis Fructus 40% ethyl alcohol extracts [GVE(1:3)] were administered p.o. once a day for 7 days, respectively. GVW(1:1) and GVW(1:3) decreased the viability of thymocytes increased by GW, but GVE(1:1) and GVE(1:3) increased the viability of thymocytes decreased by GE. GVW(1:1) and GVW(1:3) increased the viability of splenocytes decreased by GW or GE. Also, GVW(1:1) and GVE(1:1) enhanced the population of helper T cell in thymocytes, and GVE(1:1) and GVE(1:3) decreased the population of cytotoxic T cells increased by GE. Furthermore, GVW(1:1), GVW(1:3), GVE(1:1) and GVE(1:3) enhanced the population of $B220^+$ cells decreased by GW or GE, and decreased the population of $Thyl^+$ cells increased by GW or GE, and decreased the population of splenic $CD4^+$ cells increased by GW or GE. In addition, GVW(1:1) and GVW(1:3) decreased the phagocytic activity and the production of nitric oxide in peritoneal macrophages increased by GW, but GVE(1:1) and GVE(1:3) enhanced the phagocytic activity and the production of nitric oxide in peritoneal macrophages decreased by GE. These results suggest that Vitis Fructus has an regulative action on immune response of Ginseng Radix Rubra.
Keywords
Ginseng Radix Rubra; Vitis Fructus; cell viability; cell population; nitric oxide; phagocytic activity;
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