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Protective Effects of Sanyakbojungbangam-tang Ethanol Extracts on Cisplatin-induced Apoptosis in ECV304 Cells  

Kwon Kang-Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Kim Eun-Kyung (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Lee Young-Rae (Department of Biochemistry, Chonbuk National University Medical School.)
Ju Sung-Min (Department of Pathology, College of Oriental Medicine, Wonkwang University)
Ryu Do-Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Kim Sung-Hoon (Department of Pathology, College of Oriental Medicine, KyungHee University)
Jeon Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.1, 2006 , pp. 20-24 More about this Journal
Abstract
This study was designed to investigate the protective effect of Sanyakbojungbangam-tang Ethanol Extracts (SB Et-OH) on the cisplatin-induced apoptosis of human endothelial cell line ECV304 cells. After cells were treated with cisplatin, MTT assay was performed for cell viability test. To explore the mechanism of cytotoxicity, we used the several measures of apoptosis to determine whether this processes was involved in cisplatin-induced cell damage in ECV304 cells. Also, cells were treated with SB Et-OH and then, followed by the addition of cisplatin. Cisplatin decreased the viability of ECV304 cells in a dose-dependent manner and increased the caspase-3 enzyme activity ECV304 cells treated cisplatin were revealed as apoptosis characterized by nuclear staining. SB Et-OH protected ECV304 cells from cisplatin-induced nuclear fragmentation and chromatin condensation. Also, SB Et-OH inhibited the activation of caspase-3 pretense and the cleavage of poly(ADP-ribose) polymerase (PARP) in cisplatin-treated ECV304 cells. According to above results, SB Et-OH may protect ECV304 cells from the apoptosis induced by cisplatin.
Keywords
Sanyakbojungbangam-tang; ECV304 cells; caspase-3; apoptosis;
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