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Effects of Gamisingi-tang and Gamicheongpye-tang on Airway Mucus Secretion  

Kim Yun-Hee (Department of Pediatrics, College of Oriental Medicine, Daejeon University)
Kang Tak-Lim (Department of Pharmacology, College of Oriental Medicine, Daejeon University)
Han Dal-Soo (Department of Pediatrics, College of Oriental Medicine, Daejeon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.1, 2006 , pp. 156-162 More about this Journal
Abstract
In the present study, the author intended to investigate whether two oriental medical prescriptions named GSGT and GCPT significantly affect mucin release from cultured hamster tracheal surface epithelial (HTSE) cells. Confluent HTSE cells were metabolically radiolabeled with 3H-glucosamine for 24 hrs and chased for 30 min in the presen+ce of GSGT or GCPT to assess the effect of each agent on 3H-mucin release. Possible cytotoxicities of each agent were assessed dy measuring lactate dehydrogenase(LDH) release. Also, the effects of GSGT and GCPT on contractility of isolated tracheal smooth muscle were investigated. (1) GSGT did not affect mucin release without cytotoxicity ; (2) GCPT significantly stimulated mucin release from cultured HTSE cells, with significant cytotoxicity ; (3) GSGT and GCPT did not affect contractility of isolated tracheal smooth muscle. We suggest that the effects of GCPT and its components should be further investigated and it is of great value to find, from oriental medical prescriptions, novel agents which have potent expectorant effects on mucin secretion from airway goblet cells.
Keywords
airway; goblet cell; mucin; gami-singi-tang(GSGT) and gami-cheongpye-tang(GCPT);
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1 강미선, 김장현. 소아만성 재발성 호흡기 증상에 대한 고찰. 대한한방 소아과학회지. 16(2):84, 2002
2 Kim, K.C., Opaskar-Hincman, H. and Bhaskar, K.R. Secretions from primary hamster tracheal surface epithelial cells in culture. Mucin-like glycoproteins. proteoglycans and lipids. Exp. Lung Res. 15:299-314, 1989   DOI   ScienceOn
3 Kim, K.C. Possible requirement of collagen gel substratum for production of mucinlike glycoproteins by primary rabbit tracheal epithelial cells in culture. In Vitro. 21(11):617-621, 1985   DOI
4 Cross, C.E., Vandervliet, A., O'Nell, C.A., Louie, S., Halliwell, B. Oxidants, antio-xidants, and respiratory tract lining fluids. Environ. Health Perspect. 102(10):185-191, 1994   DOI
5 Freshny. Measurement of viability and cytotoxicity. In, Culture of animal cells (3rd edn), Willey-Liss, Inc. 288, 1994
6 Newhouse, M.T. and Biennenstock, J. Respiratory tract defense mechanism. In, textbook of pulmonary disease (Baum, G.L. and Wolinsky, E.(eds)), 3rd ed., Boston/Toronto, Little Brown and Company. 1983
7 江育仁, 張奇文 . 實用中醫兒科學. 上海: 上海科學技術出版社. 455461, 1995
8 許 浚. 東醫寶鑑. 서울: 法人文化社. pp 277, 280-281, 1235-1236, 1999
9 이충재 . 설치류 기관 뮤신유리 억제에서의 폴리양이온성 작용기전. 서울: 서울대학교대학원. 1997
10 Kim, K.C., Brody, J.S. Gel contraction causes mucin release in primary hamster tracheal epithelial cells growing on a collagen gel. J. Cell. Biol. 105-158a, 1987
11 金永勳 . 晴崗醫鑑. 서울: 成輔社. 448, 1995
12 Wu, R., Nolan, E. and Turner, C. Expression of tracheal differentiated function in serum-free hormone- supplemented medium. J. Cell Physiol. 125(2):167-181, 1985   DOI
13 Wu, R. and Smith, D. Continuous multiplication of rabbit tracheal epithelial cells in a defined, homone-supplemented medium. In Vitro 18(9):800-881, 1982   DOI
14 김덕곤 외 . 東醫小兒科學. 서울: 정담출판사. 28-30, 2002
15 Yu, X.-Y., Schofield, B.H., Croxton, T., Takahashi, N., Gabrielson, E.W. and Spannhake, E.W. Physiologic modulation of bronchial epithelial cell barrier function by polycationic exposure. Am. J. Respir. Cell Mol. Biol. 11:188-198, 1994   DOI   ScienceOn
16 朴炳昆 . 漢方臨床 40年. 서울: 大光文化社. 586, 1993
17 Gleich, G.J. The eosinophil and bronchial asthma: Current understanding. J. Allergy Clin. Immunol. 85(2):422-436, 1990   DOI
18 上海中醫學院 . 中醫內科學. 香港: 商務印書館. 24-25, 1982
19 Kim, K.C., Zheng, Q.X. and Van-Seuningen, I. Involvement of a signal transduction mechanism in ATP-induced mucin release from cultured airway goblet cells. Am. J. Cell Mol. Biol. 8:121-125, 1993   DOI   ScienceOn
20 Mutschler, E. and Derendorf, H. Drug actions. CRC press, Inc. Boca Raton. Florida. 410-411, 1995
21 전국한의과대학폐계내과학교실. 동의폐계내과학. 서울: 한문화사. pp 52-55, 102-114, 144-199, 2002
22 Kim, K.C., Rearick, J.I., Nettesheim, P., and Jetten, A.M. Biochemical characterization of mucous glycoproteins synthesized and secreted by hamster tracheal epithelial cells in primary culture. J. Biol. Chem. 260(7):4021-4027, 1985
23 全國韓醫科大學本草學敎授 共編著 . 本草學. 서울: 永林社. pp 136, 149, 165, 178, 193, 302, 305, 347, 349, 351, 409, 448, 460, 463, 478, 484, 537, 540, 542, 580-581, 588-589, 622, 626, 1995
24 Frigas, E., Loegering, D.A., Solley, G.O., Farrow, G.M. and Gleich, G.J. Elevated levels of the eosinophil granule major basic protein in the sputum of patients with bronchial asthma. Mayo Clin. Proc. 56(6):345-353, 1981