Browse > Article

Scavenging Activities of Reactive Oxygen and Nitrogen Species by Junglans sinensis  

Jeong Ji-Cheon (Department of Oriental Internal Medicine, College of Oriental Medicine, Dongguk University)
Bae Sung-Min (Department of Oriental Internal Medicine, College of Oriental Medicine, Dongguk University)
Shin Hyeon-Cheol (Department of Oriental Internal Medicine, College of Oriental Medicine, Daegu Hanny University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.19, no.6, 2005 , pp. 1615-1621 More about this Journal
Abstract
Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are widely implicated in the aging process and age-related diseases. The present study was carried out to investigate scavenging activities of Junglans sinensis extract and its subfraction using fluorescent probes, DCF-DA, DAF-2 and DHR 123. Jungians sinensis was washed and crushed. The crushed Junglans sinensis was extracted 3times, each time with 3 volumes of methyl alcohol at $60^{\circ}C$ for 24 h. The extract was filtered and evaporated under a reduced pressure using a rotary evaporator to yield 16 g. Scavenging activities of $ONOO^-$ was measured by Kooy' method and ROS was measured by DCFDA assay. Junglans sinensis had the marked scavenging activites of $ONOO^-$, NO and $O_2^-$. Junglans sinensis scavenged $ONOO^-$ through electron donation and dose-dependently inhibited the nitration of bovine serum albumin by $ONOO^-$. Junglans sinensis also had ROS scavenging activity. Especially, ethylacetate fraction of Junglans sinensis showed the most effective scavenging activities for ROS and RNS. These results suggest that Junglans sinensis might be developed as an effective ROS and RNS scavenger Therefore, Junglans sinensis might be used as a preventive agent for the aging and relevant to aging of illness.
Keywords
Junglans sinensis; scavenging activities; reactive oxygen species; reactive nitrogen species;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Chung, H.Y. Aging and Carcinogenic Mechanisms Induced by Free Fadicals. Kor J Gerontol., 2, pp 1-11, 1992.
2 Chung, H.Y., Kim, H.J., Jung, K.J., Yoon, J.S., Yoo, M.A., Kim, K.W., Yu, B.P. The inflammatory process in aging. Reviews in Clinical Gerontology, 10, pp 207-222, 2000.
3 江蘇新醫學院 編. 中藥大辭典, 上海科學技術出版社, 上海, p 1544-1546, 1988.
4 楊永良 主編. 中醫食料學, 中國醫藥科技出版, 北京, p 12, 1992.
5 金永海, 金甲成, 胡桃藥鋮液의 抗醒化效果에 관한 연구. 대한한의학회지, 17, pp 9-20, 1996.
6 Kooy, N.W., Royall, J.A., Ischiropoulos, H., Beckman, J.S. Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radic Res Commun., 16, pp 149-56, 1994.
7 南京中醫學院 醫經敎硏組. 黃帝內經 素問 譯釋, 上海科學技術出版社, 上海, p 4-5, 1983.
8 Choi, J.S., Chung, H.Y., Kang, S.S., Jung, M.J., Kim, J.W., No, J.K., Jung, H.A. The Structure-Activity Relationship of Flavonoids as Scavengers of Peroxynitrite. Phytother Res., 16, pp 232-235, 2002.
9 Pannala, A.S., Rice-Evans, C., Halliwell, B., Singh, S. Inhibition of peroxynitrite-mediated tyrosine nitration by catechin polyphenols. Biochem Biophys Res Commun., 232, pp 164-170, 1997.
10 Chung, H.Y., Soung, D.Y., Kim, A.R., Choi, H.R., Kim, H.J., Choi, J.S., Yang, R., Lee, K.H., Yu, B.P. Generation, Toxicity and Scavenging of ONOO : Its Involvement in the Aging Process. Kor J Gerontol., 10, pp 46-59, 2000.
11 Nagata, N., Momose, K., Ishida, Y. Inhibitory effects of catecholamines and anti-oxidants on the fluorescence reaction of 4,5-diaminofluorescein, DAF-2, a novel indicator of nitric oxide. J Biochem Tokyo, 125, pp 658-661, 1999.
12 Chung, H.Y., Kim, H.J., Kim, J.W. The inflammation hypothesis of aging: Molecular modulation by calorie restriction. Ann N Y Acad Sci., 928, pp 327-335, 2001.
13 王其飛, 王瑞廷 編著. 中醫長壽學, 遼寧科學技術出版社, 瀋陽, p 340-342, 1989.
14 이병훈, 서정철, 윤현민, 송춘호, 안창범, 장경전. 胡桃藥鋮이 Glycerol에 의한 급성신부전 유발시 뇨농축액능의 장애에 대한 영향. 대한침구학회지, 18, pp 114-122, 2001.
15 Cathcart, R., Schwiers, E., Ames, B.N. Detection of picomole levels of hydroperoxides using a fluorescent dichlorofluorescein fluorescent assay. Anal Biochem., 134, pp 111-116, 1983.
16 吳儀洛. 本草從, 上海科學技術出版社, 上海, p 203-204, 1982.
17 Chung, H.Y., Yokozawa, T., Soung, D.Y., Kye, I.S., No, J.K., Baek, B.S. Peroxynitrite-scavenging activity of green tea tannin. J Agric Food Chem., 46, pp 4484-4486, 1998.
18 Chung, H.Y., Soung, D.Y., Kim, A.R., Choi, H.R., Kim, H.J., Choi, J.S., Yang, R., Lee, K.H., Yu, B.P. Generation, Toxicity and Scavenging of ONOO-: Its Involvement in the Aging Process. Kor J Gerontol., 10, pp 46-59, 2000.
19 강형정, 장경전, 송춘호, 안창범. 胡挑水鋮이 家兎 賢臟의 抗酸化 酵素 活性에 미치는 영향. 대한침구학회지, 15, pp 473-482, 1998.
20 Carr, A.C. Oxidation of LDL by myeloperoxidase and reactive nitrogen species: reaction pathways and antioxidant protection. Arterioscler Thromb Vasc Biol., 20, pp 1716-1723, 2000.
21 Neumann, H.A., Carlsson, K., Brom, G.H. Uptake and localization of O-(beta-hydroxyethyl)-rutosides in the venous wall measured by laser scanning microscopy. Eur J Clin Pharmacol., 43, pp 423-426, 1992.
22 廣 搏, 醫學正傳, 成輔社, 서울, p 9, 1986.
23 Haenen, G.R., Paquay, J.B., Korthouwer, R.E., Bast, A. Peroxynitrte scavenging by flavonoids. Biochem Biophys Res Commun., 236, pp 591-596, 1997.
24 Park, H.J., Chung, H.Y., Kim, J., Choi, J.S. Antioxidant activity of 2,3,6-tribromo-4,5- dihydroxy benzyl methyl ether from Symphyocladia latiuscula. J Fish Sci Technol., 2, pp 1-7, 1999.
25 Yu, B.P. Aging and oxidative stress: Modulation by dietary restriction. Free Rad Biol Med., 21, pp 651-668, 1996.
26 李尙仁. 本草學, 醫藥社, 서울, p 91, 1983.
27 송주택, 정현배, 김병우, 진희성. 한국식물대보감(상권), 한국자원식물연구소, 서울, pp 98-99, 1989.
28 이경태, 송춘호. 胡桃藥鋮液이 수은(Hg)에 의한 간조직 손상에 미치는 영향. 대한침구학회지, 16, pp 221-230, 1999.