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Effect of Gamgung-tang on Fas-mediated Apoptosis in Human Thyrocytes  

Nam Kyung Soo (Intractable Disease Research Center and Department of Pharmacology, College of Medicine, Dongguk University)
Son Ok Lye (Intractable Disease Research Center and Department of Pharmacology, College of Medicine, Dongguk University)
Kim Mee Kyung (Intractable Disease Research Center and Department of Pharmacology, College of Medicine, Dongguk University)
Kim Cheorl Ho (Department of Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University)
So Myung Suk (Department of Nursing, Daegu Health College)
Jeon Byung Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University)
Shon Yun Hee (Intractable Disease Research Center and Department of Pharmacology, College of Medicine, Dongguk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.19, no.4, 2005 , pp. 934-937 More about this Journal
Abstract
Inflammatory cytokine, abundantly produced in Hashimoto's thyroiditis, induced Fas expression in normal thyrocytes. We determined that susceptibility to Fas-activated apoptosis could be influenced by inflammatory cytokine and investigated a potential role of Gamgung-tang (GGT, Glycyrrhizae Radix, black beans, Angelicae Radix and Cnidii Rhizoma) in the thyroid follicular cells. $IL-1\beta$ was able to induce Fas expression in normal thyrocytes. GGT inhibited $IL-1\beta-induced$ Fas expression. Thyroid follicular cells were found to undergo DNA fragmentation with the inflammatory cytokines. GGT inhibited DNA fragmentation in a dose-dependent manner. These results suggest that GGT inhibit Fas-mediated apoptosis in thyroid follicular cells, therefore, may have therapeutic potential in the treatment of autoimmune chronic thyroiditis.
Keywords
thyroiditis; apoptosis; Fas; cytokine; DNA fragmentation;
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