Browse > Article

Cytotoxic Effects of Radix Aconiti Extract in Lung Cancer Cell Lines  

Kwon, Kang-Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Kim, Eun-Kyung (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Moon, Hyung-Cheal (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Wonkwang University)
Song, Yung-Sun (Department of Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University)
Ryu, Do-Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.19, no.3, 2005 , pp. 628-632 More about this Journal
Abstract
The aim of this study was to investigate the cytotoxic effect and its mechanism on Radix Aconiti(RA) extract in lung cancer cell lines. RA extract treatment decreased the cell viability in a dose-dependent fashions in lung cancer cells including A549, H460, H23 and H157 cells. Many investigators reported that A549 and H460 cells expressed wild-type p53, but H23 and H157 cells preserved mutated p53. After treatment with RA extract in A549 and H460 cells, we measured the expression of p53 protein levels using Western blot. analysis. In both cells treated with RA extracts, p53 protein expressions were increased in a dose-dependent manner. In our experiments, RA extracts also have cytotoxic effects in H23 and H157, which have mutated p53. Treatment with RA extract decreased bcl-2 protein expressions in both cells. These results suggest that RA extracts have cytotoxic effects via p53 expression increase and bcl-2 inhibitable pathways in A549, H460 cells and H23, H157 cells, respectively.
Keywords
Radix Aconiti; Lung Cancer Cell Lines; p53; bcl-2; A549; H460; H23; H157;
Citations & Related Records
연도 인용수 순위
  • Reference
1 대한병리학회. 병리학, 서울, 高文社, pp 201-202, 1997.
2 陳存仁. 圖說漢方醫藥大解典(第3卷),東京, 講談社, pp 268-271, 1982.
3 申佶求. 申氏本草學名論, 서울, 壽文社, P 23-29, 1982.
4 Pruschy, M., Rocha, S. Key targets for the execution of radiation-induced tumor cell apoptosis: the role of p53 and caspases, Int. J. Radiat. Oncol. BioI. Phys. 49(2):561-567, 2001.
5 Condorelli, G., Morisco, C. Stassi, G., Notte, A., Farina, F., Sgaramella, G., de Rienzo, A., Roncarati, R., Trimarco, B., Lembo, G. Increased cardiomyocyte apoptosis and changes in proapoptotic and antiapoptotic genes bax and bcl-2 during left ventricular adaptations to chronic pressure overload in the rat. Circulation 99(23):3071-3078, 1999.
6 金鍾大 외 12인. 東醫師系內科學, 서울, 도서출판 한 문화사,P 79, 2002.
7 中華人民共知國衛生部藥典委員會. 中華人民共和國藥典(一部), 北京, 人民衛生出版社, P 157, 158, 1985.
8 송계용 外. 핵심 병리학, 서울, 고려의학, P 147, 1998.
9 Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Analatycal Biochemistry 72, 248-254, 1976.
10 Brady, H.J., Gil-Gomez, G. Bax. The pro-apoptotic Bcl-2 family member, Bax. International Journal of Biochemical Cell Biology 30(6):647-650, 1998.
11 Zeimet, A.G., Riha, K. New insights into p53 regulation and gene therapy for cancer, Biochemical Pharmacology 60(8):1153-1163, 2000.
12 Tudor, G., Aguilera, A., Halverson, D.O., Laing, N.D., Sausville, E.A. Susceptibility to drug-induced apoptosis correlates with differential modulation of Bad, Bcl-2 and Bcl-xl, protein levels. Cell Death & Differentiation 7(6):574-586, 2000.
13 趙鍾寬. 한방임상종양학, 대전, 周珉出版社,pp 725-758, 2001.
14 Cho, J.H., Ahn, KS., Kim, J.G., Hons, Y.S. Enhanced induction of Bax gene expression in H460 and H1299 cells with the combined treatment of cisplatin and adenovirus mediated wt-p53 gene transfer. Experimental and Molecular Medicine 32(1):23-28, 2000.
15 Kroemer, G. The proto-oncogene Bcl-2 and its role in regulating apoptosis. Nature Medicine 3(6):614-620, 1997.   DOI   ScienceOn
16 辛民敎. 原色臨床本草學, 서울, 南山堂, pp 263-265, 1986.
17 繆希雍. 神農本草經疏, 中國醫學大系(43卷),서울, 羅江出版社, pp 579-582, 1986.
18 許浚. 東醫寶鑑, 서울, 南山堂, P 384, 446, 732, 1980.
19 Yeudall, W.A., Paterson, I.C, Patel, V., Prime, S.S. Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53. European Journal of Cancer B oral oncology 31B(2):136-143, 1995.
20 Hattori, T., Ookawa, N., Fujita, R, Fukuchi, K. Heterodimerization of Bcl-2 and Bcl-X(L) with Bax and Bad in colorectal cancer. Acta Oncologica 39(4):495-500, 2000.
21 Vander, Heiden, M.G., Thompson, C.B. Bcl-2 proteins : regulators of apoptosis or of mitochondrial homeostasis? Nature Cell Biology 1(8):E209-216, 1999.
22 Pellizzaro, C., Coradini, D., Daniotti, A., Abolafio, G., Daicone. M.G. Modulation of cell cycle-related protiein expression by sodium butyrate in human non-small cel lung cancer cell lines. International Journal of Cancer 91, 654-657, 2001.
23 권강범, 김은경, 정은실, 심정섭, 김강산, 신병철, 송용선, 류도곤. HepG2 간암세포에 대한 附子추출물의 고사 유도 효과. 대한한의학회지 25(2):33-40, 2004.
24 朴炳昆. 韓方臨床40年, 서울, 大光文化社, P 82, 1981.
25 Masato, M, Hideki, I., Yasuo, M Phosphorylation of p53 protein in A549 human pulmonary epithelial cells exposed to asbestos fibers. Environmental Health Perspectives 111(4):509-512, 2003.   DOI   ScienceOn
26 최정신, 김정혜. 최신병리학, 서울, 도서출판 정담, P 117, 1996.
27 권강범, 김은경, 정은실, 황인진, 김우경, 심정섭, 김강산, 신병철, 송용선, 류도곤. HepG2 간암세포주기에 대한 附子 추출물의 효과. 동의생리병리학회지 18(2):427-430, 2004.
28 高本劉. 新編中藥大解典, 台北, 新文豊出版公司, (上冊) pp 270-274,322,545, (中冊) pp 1166-1169, 1984.
29 Weller, M. Caspase-dependent chemotherapy-induced death of glioma cells requires mitochondrial cytochrome c release, Biochemical Biophysical Research Communication 281(2):3242-3247, 2001.