Browse > Article

Evaluation of Safety with Gamgung-tang Using Rec assay and Enzymatic Methods  

Shon, Yun-Hee (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University)
Baek, Tae-Seon (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University)
Moon, Ji-Sun (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University)
Kim, Mee-Kyung (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University)
Kim, Cheorl-Ho (Department of Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University)
Jeon, Byung-Hun (Department of Pathology, College of Medicine, Wonkwang University)
Nam, Kyung-Soo (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.19, no.1, 2005 , pp. 98-101 More about this Journal
Abstract
Gamgung-tang(GGT) that is included in Gamdu-tang(consists of Glycyrrhizae Radix, black beans) and Gunggui-tang(consists of Angelicae Radix and Cnidii Rhizoma), showed therapeutic effects of autoimmume thyroiditis in the previous reports. GGT was tested for the safety using Rec assay and enzymatic methods. In the Rec assay, Bacillus subtilis H-17$(Rec^+)$ and M-45$(Rec^-)$ strains were used to test DNA damage activity. From the results, there was no DNA damage of GGT. Hepatotoxicity of GGT to female ICR mice was also monitored by the measurements of serum(s)-GOT, s-GPT and LDH activities after oral feeding for 15 days. GGT was not shown any significant changes of s-GOT, s-GPT and LDH activities in mice sera.
Keywords
Gamgung-tang; DNA damage; Rec assay; Hepatotoxicity;
Citations & Related Records
연도 인용수 순위
  • Reference
1 최호승, 김영목, 임종국, 손윤희, 남경수, 김철호, 전병훈. 감궁탕 가미방이 갑상샘 기능장애에 미치는 효과. 동의생리병리학회지 17, 648-655. 2003.
2 남경수, 손윤희, 백태선, 김철호, 임종국, 황철원. Antithyroglobulin monoclonal antibody의 제작 및 특성. 한국생명과학회지 12, 460-463, 2002.
3 錢百炎. 中草藥注射劑, p.71-130, 上海科學技術出版社, 中國, 1981.
4 Shon, Y.H., Lee, H.S., Lim, J.K., Kim, C.H., Jeon, B.H. and Nam, K.S. Effect of herbal medicines on cytokine-induced cytotoxicity and MHC class II antigen expression in rat thyroid cells. Biol. Pharm. Bull. 27, 371-374, 2004.
5 손윤희, 김철호, 전병훈, 남경수. Thyroglobulin에 대한 단일클론항체의 혈소판응집 저해작용, 동의생리병리학회지 18, 534-537, 2004.
6 Shon, Y.H. , Lee, J.S., Lee, H.W. and Nam, K.S. Antimutagenic potential of Phellinus igniarius. Journal of Microbiology and Biotechnology 9, 525-528, 1999.
7 Shon, Y.H. and Nam, K.S. Antimutagenicity and induction of anticarcinogenic phase II enzymes by basidiomycetes. J of Ethnopharmacology 77, 103-109, 2001.
8 Kim, S.Y., Shon , Y.H., Lee, J.S., Kim, C.H, and Nam, K.S. Antimutagenic activity of soybeans fermented with basidiomycetes in Ames/Salmonella test. Biotechnology letter 22, 1197-1202, 2000.
9 Nam, K.S., Choi, Y.R. and Shon , Y.H. Evaluation of the antimutagenic potential of chitosan oligosaccharide : Rec, Ames and Umu assays. Biotechnology letters 23, 971-975, 2001.
10 Shon, Y.H., Ha, Y.M., Jeong, T.R., Kim, C.H. and Nam, K.S. Antimutagenic effects of chitosan oligosaccharides 2-amino-3,8-dimethylimidazo[4,5-f] quinoxaline(MeIQx). J. Biochem. Molecular Biology 34, 90-94, 2001.
11 回島疆太郎, 賀田恒夫, 近藤宗平, 外村晶. 環境變異原實驗法, 47-69, 講談社, 東京, 1980.
12 Shon, Y.H., Kim, S.Y., Lee, J.S., Lim, J.K. and Nam, K.S. Antimutagenic effects of soybeans fermented with basidiomycetes 2-amino-3,8-dimethylimidazo [4,5-f] quinoxaline (MeIQx). Journal of Microbiology and Biotechnology 11, 346-349, 2001.
13 Moon, S.K. Kang, B.S., Kim, Y.G., Chung, T.W., Kim, J.R., Cha, B.Y., Moon, J.Y., Lim, J.K., Shon, Y.H. , Nam, K.S., Ko, J.H. , Jeon, B.H. and Kim , C.H. Induction of an autoimmune thyroiditis by deglycosylated thyroglobulin induction of a Tel-mediated experimental autoimmune thyroiditis by deglycosylated porcine thyroglobulin. Immunopharmacology and Immunotoxicology 26, 355-372, 2004.
14 Kada, T., Tutikada, K. and Sadale, Y. In vitro and hostmediated 'rec-assay' procedures for screening chemical mutagens: and phloxine. A mutagenic Red dye detected. Mutat. Res. 16, 165-174, 1972.