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Cytoprotective Effects of Radix Curcumae Aromaticae in Human Umbilical Vein Endothelial Cells  

Seo Eun A (Department of Food and Nutrition, School of Human Envioronmental Science, Wonkwang University)
Chung Hun Taeg (Genome Research Center for Immune Disorders, School of Medicine, Wonkwang University)
Ko Kwang Hak (Department of Physiology, School of Oriental Medicine, Wonkwang University)
Kwon Kang Beom (Department of Physiology, School of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.6, 2004 , pp. 1805-1809 More about this Journal
Abstract
In order to validate the use of Radix Curcumae Aromaticae as an anti-inflammatory drug in the traditional Korean medicine, I have investigated the effect of water-soluble extract of Radix Curcumae Aromaticae (ECA) on the expression of inducible heme oxygenase-1 (HO-1), which ha.s anti-inflammatory and cytoprotective effects stimulates, in human umbilical vein endothelial cells (HUVECs) stimulated with a high dose of pro-inflammatory tumor necrosis factor-alpha (TNF-α). The extract protected dose-dependently HUVECs against TNF-α-induced apoptosis, as measured qualitatively by a nuclear staining method using the fluoresoence DAPI and quantitatively by a flow cytometry using fluoresce-enhanced Annexin V antibody, and significantly Increased HO-1 expression, as determined by Western blotting analysis using anti-HO-1 antibody. Biockage of HO-1 activity by a pharmacological inhibitor reversed cytoprotection afforded by the extract, and treatment with carbon monoxide, one of HO-1 metabolites, resulted in cytoprotection comparable to the extract. These results suggest that ECA may have therapeutic potential in the control of endothelial disorders caused by inflammatory cytokines.
Keywords
Radix Curcumae Aromaticae; cytokines; tumor necrosis factor-alpha Human umbilical vein endothelial cells; Heme Oxygenase-1;
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