Browse > Article

Protective Effects of Hwangryunhaedog-tang on Hypoxia-induced Apoptosis in H9c2 Cardiomyoblast Cells  

Jeong Jae Eun (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Yu Bong Seon (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Park Jin Yeong (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Jeon In Cheol (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Park Sang Beom (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Lee Dae Yong (Department of 3rd Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
Lee Min Goo (Department of 3rd Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
Lee In (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Moon Byun Soon (Department of 3rd Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.6, 2004 , pp. 1733-1739 More about this Journal
Abstract
The water extract of Hwangryunhaedog-tang(HRHDT} has been traditionally used for treatment of ischemic heart and brain damage in oriental medicine. However, little is known about the mechanism by which the water extract of HRHDT rescues cells from these damages. This study was designed to investigate the protective mechanisms of HRHDT on hypoxia-induced cytotoxicity in H9c2 cardiomyoblast cells. Hypoxia, markedly decreased the viability of H9c2 cells, which was characterized with apparent apoptptic features such as chromatin condensation as well as fragmentation of genomic DNA and nuclei. However, HRHDT significantly reduced hypoxia-induced cell death and apoptotic characteristics. Also, HRHDT prevented the mitochondrial dysfunction including the disruption of mitochondria membrane permeability transition (MPT) and an increase in expression of anti-apoptotic Bcl-2 proteins in hypoxia-H9c2 cells. Taken together, this study suggests that the protective effects of the water extract of HRHDT against hypoxic damages may be mediated by the modulation of Bcl-2 and Bak expression.
Keywords
Hwangryunhaedog-tang; hypoxia; H9c2; MPT; anti-apoptotic Bcl-2 proteins;
Citations & Related Records
연도 인용수 순위
  • Reference
1 /
[ 丹羽靭負 ] / 활성산소를 다스리면 무병장수 할 수 있다
2 /
[ 鞠潤範 ] / 黃連解毒湯이 자발적 고혈압 백서의 혈압 및 신장기능에 미치는 영향
3 /
[ 金福海 ] / 黃連解毒湯 合 六味地黃湯 加 釣鉤藤이 자발적 고혈압 백서의 혈압 및 신장기능에 미치는 영향
4 대황황련해독탕의 항고지혈증 작용 /
[ 김동현(外) ] / 생약학회지
5 /
[ 孫昌鳳 ] / 抗菌活性作用에 대한 黃連解毒湯의 효능에 관한 연구
6 /
[ 尹星燦 ] / 黃連解毒湯加味方에 의한 면역글로블린E 매개성 아나필락시스의 억제
7 /
[ 新谷太 ] / Pathophysiology로 이해하는 내과학 (3卷)
8 /
[ 洪元植(譯) ] / 黃帝內經素問
9 HSP60, Bax, and Cardiac Apoptosis /
[ Knowlton AA;Gupta S ] / Cardiovasc Toxicol
10 Mechanism controlling cellular suicide: role of Bcl-2 and caspases /
[ Allen RT;Cluck MW;Agrawal DK ] / CMLS
11 Monoclonal antibody-mediated tumor regression by induction of apoptossis /
[ Trauth BC;Klas C;Peters A MJ;Matzuku S;Moller P;Falk W;Debatin KM;Krammer PH ] / Science
12 /
[ 葛洪 ] / 주後備急方
13 Measurement of superoxide-derived free radicals in the reperfused heart. Evidence for a free radical mechanism of reperfusion injury /
[ Zweier JL ] / J. Biol. Chem.
14 Demonstration of free radical generation in stunned myocardium of intact dogs with the use of the spin trap alpha-phenyl N-tert-butyl nitrone. /
[ Bolli, R;Patel, BS;Jeroudi, MO;Lai, EK;McCay, PB ] / J. Clin. Invest.
15 Canine myocardial reperfusion injury. Its reduction by the combined administration of superoxide dismutase and catalase /
[ Jolly SR;Kane WJ;Bailie MB;Abrams GD;Lucchesi BR ] / Circ Res
16 Effect of hyperbaric oxygen on apoptosis in neonatal hypoxia- ischemia rat model /
[ Calvert JW;Zhou C;Nanda A;Zhang JH. ] / J Appl Physiol
17 Constituvely enhanced nbl expression is associated with the induction of inter-nucleosomal DNA cleavage by actinomycin D /
[ Naora, H;Nishida, T;Shindo, Y;Adachi, M;Naora, H ] / Biochem. Biophys. Res. Comm.
18 Prooxidant states and tumor promotion /
[ Cerutti PA ] / Science
19 /
[ 王燾 ] / 外臺秘要
20 /
[ 王氷(註) ] / 注解補注 黃帝內經 素問
21 /
[ 이원로(編著) ] / Clinical Cardiology
22 Protective effects of flavonoids in the roots of Scutellaria baicalensis Georgi against hydrogen peroxide-induced oxidative stress in SH-SY5Y cells /
[ Gao Z;Huang K;Xu H ] / Pharmacol Res
23 Protection aganist oxidants in biological systems: the superoxide theory of oxygen toxicity /
[ Halliwell, B;Gutteridge, JMC ] / Free radicals in biology and medicine
24 Death by design. Programmed cell death in cardiovascular biology and disease /
[ MacLellan WR;Schneider MD ] / Cir Res
25 Predominant Role of Hypoxia-Inducible Transcription Factor (Hif)-1alpha versus Hif-2alpha in Regulation of the Transcriptional Response to Hypoxia /
[ Sowter HM;Raval R;Moore J;Ratcliffe PJ;Harris AL ] / Cancer Res