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Anti-metastatic and Anti-angiogenic Activities of Ekong-san and Its Metabolites by Human Intestinal Bacteria  

Kang Chang Hee (Department of Oriental Medicine, Kyung Hee University)
Myung Eu Gene (Department of Oriental Medicine, Kyung Hee University)
Kang Hee (Department of Oriental Medicine, Kyung Hee University)
Choi Sun Mi (Korea Institute of Oriental Medicine)
Shim Bum Sang (Department of Oriental Medicine, Kyung Hee University)
Kim Sung Hoon (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University)
Choi Seung Hoon (Department of Oriental Medicine, Kyung Hee University)
Shin Hyeun Kyoo (Korea Institute of Oriental Medicine)
Kim Dong Hyun (College of Pharmacy, Kyunghee University)
Ahn Kyoo Seok (Department of Oriental Medicine, Kyung Hee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.6, 2004 , pp. 1686-1693 More about this Journal
Abstract
Ekong-san(EKS) was expected to have inhibitory effects on angiogenesis, considering the fact that its constituents such as Ginseng Radix, Glycyrrhizae Radix and Citri Pericarpium were reported to inhibit angiogenesis. Moreover, recently several metabolites transformed by the human intestinal microflora were reported to enhance effectiveness compared to their crude drugs. Based on these data, this study was designed to confirm whether the EKS metabolites (EKS-M) can significantly exert the anti-angiogenic and anti-metastatic activites. Hence, with EKS and EKS-M, viability assay, proliferation assay, in vitro tube formation assay, gelatin zymogram assay, in vitro invasion assay were carried out. EKS showed less toxicity in ECV304 and HT1080 cells than EKS-M. EKS-M inhibited the proliferation of HT1080 cells by 30% at 200㎍/㎖ and 42% at 400 ㎍/㎖ respectively. Also, EKS-M degraded the tube network at 200㎍/㎖. EKS and EKS-M inhibited the expression of MMP-9 at 200 and 400㎍/㎖ in HT1080 cells. EKS reduced the invasive activity of HT1080 cells through matrigel coated transfilter atthe concentration of 200㎍/㎖ more effectively than EKS-M. These data suggest that EKS and EKS-M has anti-angiogenic and anti-metastatic activities.
Keywords
Ekong-san; Ekong-san metabolite; human intestinal microflora; angiogenesis; metastastasis;
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