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In Vitro Inhibition of Pro-inflammatory Mediator mRNA Expression by Nephrite in Lipopolysaccharide-induced Mouse Macrophage Cells  

Yeom Mi-jung (Department of Acupuncture & Meridian, Graduate School of East-West Medical Science, Kyunghee University)
Choi Bo-Hee (Bokyung Nephrite Co.,)
Han Dong-Oh (Department of Acupuncture & Meridian, Graduate School of East-West Medical Science, Kyunghee University)
Lee Hye-Jung (Department of Acupuncture & Meridian, Graduate School of East-West Medical Science, Kyunghee University)
Shim Insop (Department of Acupuncture & Meridian, Graduate School of East-West Medical Science, Kyunghee University)
Kim Sung-Hoon (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University)
Hahm Dae-Hyun (Department of Acupuncture & Meridian, Graduate School of East-West Medical Science, Kyunghee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.6, 2004 , pp. 1622-1627 More about this Journal
Abstract
Nephrite has been widely used as a medicinal mineral resource to treat a numerous chronic diseases and to replenish vital essence and blood in the Korean traditional medicine. However, as of yet, there is little understanding of the pharmacological and biochemical mechanisms of its therapeutic effects as regards anti-inflammation. We therefore examined whether nephrite represses the expression of major inflammation mediators, such as interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2), in LPS-stimulated murine macrophage cell line, RAW264.7 by using RT-PCR. The powder suspension and water extracts of nephrite significantly inhibited the mRNA expression of the mediators, despite a little toxic effects on growth of RAW 264.7 cells within the concentration range tested. These experimental results suggested that the nephrite can be utilized as a functional mineral exerting the anti-inflammation medicinal effect.
Keywords
nephrite(軟玉); inflammation; pain; IL-1β; TNF-β; COX-2; iNOS;
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[] / 광업진흥 1993년 신년호