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Study on Anti-thrombotic Activity, Superoxide Generation in Human Neutrophils and Platelet Aggregation in Human Blood of Hwao-tang  

Park Won Hwan (Department of Diagnostics, College of Oriental Medicine, Deongguk University)
Park Soo Young (Department of Diagnostics, College of Oriental Medicine, Deongguk University)
Park Tae Woo (Department of Diagnostics, College of Oriental Medicine, Deongguk University)
Kim Jong Gu (Department of Diagnostics, College of Oriental Medicine, Deongguk University)
Kim Seog Ha (Department of Diagnostics, College of Oriental Medicine, Deongguk University)
Kim Cherl Ho (Department of Biochemistry, College of Oriental Medicine, Dongguk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.5, 2004 , pp. 1494-1504 More about this Journal
Abstract
The present paper reports the effects of Hwaotang an atherosclerosis using a spontaneous experimental model, We have also investigated the pharmacological effect of Hwaotang on collagen- and ADP-induced blood platelet aggregation, thrombin-induced conversion of fibrinogen and fibrinolysis in in vitro experiments, and various effects on stimuli-induced superoxide generation in human neutrophils. Hwao-tang was shown to have inhibitory effect on collagen- and ADP-induced blood platelet aggregation, on thrombin-induced conversion of fibrinogen to fibrin and on the activity of plasminogen or plasmin. Hwao-tang also significantly inhibited fMLP-induced superoxide generation in a concentration-dependent manner, but not that induced by arachidonic acid. Hwao-tang inhibited neutrophil functions, including degranulation, superoxide generation, and leukotriene B4 production, without any effect on 5-lipoxygenase activity. In conclusion, the protection of extracts of Hwao-tang on the ischemic infarction induced artificially might be involved to their inhibition of thrombotic action. The results also indicate that Hwao-tang exerts the effects on superoxide generation related to the inhibition of neutrophil functions.
Keywords
Hwao-tang(huayutang, 化瘀湯); thrombosis; activating blood circulation and eliminating blood stasis; aggregation; atherosclerosis; superoxide generation; neutrophils;
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  • Reference
1 /
[ Ming, O. ] / Chinese-English manual of common-used prescriptions in TCM
2 Selectivity of neutrophil 5-lipoxygenase and cyclo-oxygenase inhibition by an anti-inflammatory flavonoid glycoside and related aglycone flavonoids /
[ Moroney, M.A.;Alcaraz, M.J.;Forder, R.A.;Carey, F.;Hoult, J.R.S. ] / J. Pharm. Pharmacol.
3 Selective inhibition of arachidonate 5-lipoxygenase by novel acetohydroxamic acids: biochemical assessment in vitro and ex vivo /
[ Tateson, J.E.;Randall, R.W.;Reynolds, C.H.;Jackson, W.P.;Bhattacherjee, P.;Salmon, J.A.;Garland, L.G. ] / Br. J. Pharmacol.
4 /
[ J.G.,Kim ] / clinic lipid
5 /
[ S.G.,Seo ] / Adult & Aged disease
6 /
[ A.D.T.Govan;P.S. Macfarlane;R.Callander;J. D., Lee(trans) ] / Pathology
7 Studies on Scutellaria Radix (VIII) Anti-thrombic actions of 70% methanolic extract and various fractions from Scutellariae Radix /
[ Kubo, M.;Matsuda, H. ] / Shoyakugaku Zasshi
8 Recombinant tissue plasminogen activator prevents intimal hyperplasia after balloon angioplasty in hypercholesterolemic rabbits /
[ Kanamasa, K.;Ishida, N.;Kato, H.;Sakamoto, S.;Nakabayashi, T.;Otani, N.;Ishikawa, K.;Katori, R. ] / Jpn. Circulation Journal
9 /
[ M.G.,Shin ] / Clinic Herbs Science
10 /
[ 久保道德;松田秀秋;松田玲子 ] / 抗血栓形成作用について, 牧丹皮の硏究 第8報
11 A study of the influence of both of Shunqidaotantang and Huayutang on thrombosis, contusion-hyperemia, and hyperlipidemia /
[ W. H., Park(etc.) ] / The J.of DGOM
12 /
[ Peng Pu In ] / Great dictionary of prescription in traditional chinise medicine, beijing
13 /
[ Zhang Enqin ] / Clinic of Traditional Chinese Medicine (Ⅰ) in A Practical English-Chinese Library of TCM
14 Effects of a Korean traditional formulation, Hwaotang, on superoxide generation in human neutrophiles, platelet aggregation in human blood, and nitric oxide, prostaglandin E2 production and paw oedema induced by carrageenan in mice /
[ Park WH;Park SY;Kim HM;Kim CH ] / Immunopharmacol Immunotoxicol
15 Inhibitory effets of Hwaotang on the atherosclerosis and the venous thrombosis /
[ M.Y. Hong ] / Thesis for the degree of Doctor
16 The O2- forming NADPH oxidase of the phagocytes: nature, mechanisms of activation and function /
[ Rossi, F. ] / Biochim Biophys Acta
17 Luminol chemi- luminescence and active oxygen generation by activated neutrophils /
[ Takahashi, R.;Edashige, K.;Sato, E.F.;Inoue, M.;Matsuno, T.;Utsumi, K. ] / Arch Biochem Biophys
18 Effect of cystathionine ketimine on the stimulus coupled responses of neutrophils and their modulation by various protein kinase inhibitors /
[ Zhang, J.;Sugahara, K.;Sagara, Y.;Fontana, M.;Dupre, S.;Kodama, H. ] / Biochem Biophys Res Commun
19 Different effect of diastereoisomers of cystathionine sulfoxide on the stimulus coupled responses of human neutrophils /
[ Shimazaki, Y.;Zhang, J.;Wakiguchi, H.(et al.) ] / Biochem Biophys Res Commun
20 Effects of cystathionine and cystathionine metabolites on the phosphorylation of tyrosine residues in human neutrophils /
[ Zhang, J.;Sagara, Y.;Fontana, M.;Dupre, S.;Cavallini, D.;Kodama, H. ] / Biochem Biophys Res Commun
21 Platelet aggregation inhibitors from the seeds of Swietenia mahagoni: inhibition of in vitro and in vivo platelet-activating factor-induced effects of tetranortriperpenoids related to swietenine and swietenolide /
[ Ekimoto, H.;Irie, Y.;Araki, Y.;Han, G.Q.;Kadota, S.;Kikuchi, T. ] / Planta Med.
22 New mutant rabbit strain with hypercholesterolemia and atherosclerotic lesions produced by serial inbreeding /
[ Kurosawa, T.;Kisanagi, M.;Yamasaki, Y.;Senga, Y.;Yamamoto, T. ] / Laboratory Animal Science
23 Probucol prevents the progression of atheroclerosis in Watanabe heritable hyperlipidemic rabbit, an animal model for familial hypercholesteromoa /
[ Kita, T.;Nagano, Y.;Yokode, M.;Ishii, K.;Kume, N.;Ooshima, A.;Yoshida, H.;Kawai, C. ] / Proc. Natl. Acad. Sci. USA
24 Triphenyltin fluoide in vitro inhibition of rabbit platelet collagen-induced aggregation and ATP secretion and blockage of arachidonic acid mobilization from membrane phospholipids /
[ Manabe, S.;Wada, O.;Matsui, H.;Takeda, M.;Kobayashi, N.;Maekawa, T. ] / Biochem. Pharmacology
25 The fibrin plate method for estimating fibrinolytic activity /
[ Astrup, T.;Mullertz, S. ] / Arch. Biochem. Biophys.
26 Chlorination of taurine by human neutrophils. Evidence for hypochlorous acid generation /
[ Weiss, S.J.;Klein, R.;Slivka A.;Wei, M. ] / J Clin Invest
27 The role of leukotrienes in inflammation /
[ Henderson, W.R. ] / Ann. Intern. Med.
28 Isolation of human salivary polymorphonuclear leukocytes and their stimulation-coupled responses /
[ Yamamoto, M.;Saeki, K.;Utsumi, K. ] / Arch Biochem Biophys
29 A study of the novel anti-inflammatory agent florifenine. Topical anti-inflammatory activity and influence on arachidonic acid metabolism and neutrophil functions /
[ Bustos, G.;Ferrandiz, M.L.;Sanz, M.J.;Paya, M.;Alcaraz, M.J. ] / Naunyn-Schmiedeberg's Arch. Pharmacol.
30 Tetrahydrobiopterin synthesis. An absolute requirement for cytokine-induced nitric oxide generation by vascular smooth muscle /
[ Gross, S.S.;Levi, R. ] / J. Biol. Chem.
31 Variabilin: a dual inhibitor of human secretory and cytosolic phospholipase A2 with anti-inflammatory activity /
[ Escrig, V.;Ubeda, A.;Ferrandiz, M.L.;Darias, J.;Sanchez, J.M.;Alcaraz, M.J.;Paya, M. ] / J. Pharmacol. Exp. Ther.
32 Priming effect of N-acetyl-S-(3-oxo-3-carboxy-n-propyl) cysteine in human neutrophils and tyrosyl phosphorylation of 45 kDa protein /
[ Ito, O.;Zhang, J.;Zhang, M. ] / Clin Chim Acta
33 Surface-reactive stimuli selectively increase protein phosphorylation in human neutrophils /
[ Schneider, C., Zanetti, M. and Romeo, D. ] / FEBS Lett
34 In vitro responses of human neutrophils to N-formyl-methionyl-leucyl-phenylalanine: correlation with effects of acute bacterial infection /
[ McCall, C.E.;Bass, D.A.;Dechatelt, L.R.;Link, A.S.;Mann, M. ] / J Infect Dis