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Effects of Dangguijakyak-san Extract on Nitric Oxide Production and Cytokine Gene Expression in RAW 264.7 Macrophages Cell  

Shin Sang Woo (Department of Pathology, College of Oriental Medicine, Daegu Haany University)
Lee Young Sun (Department of Pathology, College of Oriental Medicine, Daegu Haany University)
Park Jong Hyun (Department of Pathology, College of Oriental Medicine, Daegu Haany University)
Kwon Taeg Kyu (School of Medicine, Keimyung University)
Suh Seong Il (School of Medicine, Keimyung University)
Kwon Young Kyu (Department of Physiology, College of Oriental Medicine, Daegu Haany University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.5, 2004 , pp. 1443-1448 More about this Journal
Abstract
The present study was conducted to evaluate the immunomodulatory effects of Dangguijakyak-san(당귀작약산). We investigated the effects of cell proliferation in mouse spleen cell and RAW 264.7 macrophages cells. Dangguijakyak-san enhanced mitogenic activity in the dose-response manner in mouse spleen cells and RAW 264.7 macrophages cells. In nitric oxide (NO) synthesis and iNOS mRNA expression by Dangguijakyak-san, Dangguijakyaksan alone had an effect on NO synthesis and iNOS mRNA expression in RAW 264.7 cells. NO production and iNOS mRNA expression which is excessively induced by LPS decreased after treatment of Dangguijakyak-san. The expressions of cytokine gene by Dangguijakyak-san investigated using reverse transcription polymerase chain reaction (RT-PCR). In RT-PCR, IL-1α, IL-1β and IL-6 mRNA expressions induced in Dangguijakyak-san-treated RAW 264.7 cells. These data indicate that 1) Dangguijakyak-san can modulate various immune response and 2) the immunomodulatory effects of Dangguijakyak-san may be, in part, associated with the regulation of NO synthesis, the expressions of these cytokine as well as the mitogenic effect on spleen cells and macrophages cells.
Keywords
Dangguijakyak-san(當歸芍藥散); cell proliferation; NO; iNOS; cytokine;
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  • Reference
1 Nitric oxide-scavenging and antioxidant effects of Uraria crinita root /
[ Gow-Chin Yen;Hsi-Huai Lai;Hsin-Yi Chou. ] / Food Chemistry
2 IL-6 mRNA Expression in Mouse Peritoneal Macrophages and NIH3T3 Fibroblasts in Response to Candida albicans /
[ Lee YS;Kim HS;Kim SK;Kim SD. ] / J Microbiol Biotechnol.
3 A traditional Chinese herbal medicine, ren-shen-yang- rong-tang (Japanese name: ninjin-yoei-to) augments the production of granulocyte-macrophage colony-stimulating factor from human peripheral blood mononuclear cells in vitro /
[ Okamura S;Shimoda K;Yu LX, Omori F;Niho Y. ] / Int J Immunopharmacol.
4 마이토젠 및 림포카인이 마우스 비장 세포 임파구 및 사람 말초 혈액 임파구 증식 반응에 미치는 영향 /
[ 정태준;김정목;조양자;정용훈;임대철 ] / 대한면역학회지
5 Capsaicin 전처치가 마우스 대식세포의 기능에 미치는 영향 /
[ 박재승;이정호;하대유 ] / 대한면역학회지
6 Membrane potential modulates release of tumor necrosis factor in lipopolysaccharide-stimulated mouse macrophages /
[ Haslberger A;Romanin C;Koerber R. ] / Mol Biol Cell
7 Stimulation of interleukin-6 and prostaglandin E2 secretion from peritoneal macrophages by polymers of albumin /
[ Shacter E;Arzadon GK;Williams JA. ] / Blood
8 /
[ 尹用甲 ] / 東醫方劑와 處方解說
9 /
[ 張仲景 ] / 仲景全書
10 /
[ 金楨氾 ] / 金궤要略辨釋
11 /
[ 許浚 ] / 東醫寶鑑
12 Polygonum tinctorium extract suppresses nitric oxide production by activated macrophages through inhibiting inducible nitric oxide synthase expression /
[ Ishihara T;Okura T;Kohno K;Tanimoto T;Ikegami H;Kurimoto M. ] / J Ethnopharmacol
13 Suppression of inducible nitric oxide synthase expression in RAW 264.7 macrophages by two-carboline alkaloids extracted from Melia azedarach /
[ Lee BG;Kim SH;Zee OP;Lee KR;Lee HY;Han JW;Lee HW. ] / Eur J Pharmacol
14 황기가 면역세포의 기능에 미치는 영향 /
[ 송봉근;이언정;김형균;진선두;김성재;김동혁 ] / 대한본초학회지(본초분과학회지)
15 The aqueous extract of Rhodiola sachalinensis root enhances the expression of inducible nitric oxide synthase gene in RAW264.7 macrophages /
[ Seo WG;Pae HO;Oh GS;Kim NY;Kwon TO;Shin MK;Chai KY;Chung HT. ] / J Ethnopharmacol
16 Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS-activated RAW264.7 cells, a murine macrophage cell line /
[ Kawamata H;Ochiai H;Mantani N;Terasawa K. ] / Am J Chin Med
17 Inhibitory effect of ethyl acetate fraction from Cudrania tricuspidata on the expression of nitric oxide synthase gene in RAW 264.7 macrophages stimulated with interferon-and lipopolysaccharide /
[ Seo WG;Pae HO;Oh GS;Chai KY;Yun YG;Kwon TO;Chung HT. ] / Gen Pharmacol
18 Camptothecin suppresses nitric oxide biosynthesis in RAW 264.7 macrophages /
[ Chiou WF;Chou CJ;Chen CF. ] / Life Sci
19 신수혈의 침자극과 황기약침이 실험용 생쥐의 면역활성물질인 cytokine의 IL-6 발현에 미치는 영향 /
[ 김종수;신상습;김철호;박선동;박원환 ] / 대한침구학회지
20 Differences in immunomodulating effects between wild and cultured Panax ginseng /
[ Mizuno M;Yamada J;Terai H;Kozukue N;Lee YS;Tsuchida H. ] / Biochem Biophys Res Commun
21 인삼 사포닌이 인간면역계 사이토카인 유전자의 발현에 미치는 영향 /
[ 박종욱;한인숙;서성일;백원기;서민호;배지현;최병길 ] / 고려인삼학회지
22 Inhibitory effects of butanol fraction of the aqueous extract of Forsythia koreana on the nitric oxide production by murine macrophage-like RAW 264.7 cells /
[ Kim NY;Kang TH;Song EK;Pae HO;Chung HT;Kim YC. ] / J Ethnopharmacol
23 산화질소(Nitric Oxide) 이로운가? 해로운가? : 산화질소의 화학과 응용 /
[ 황광진 ] / 대한화학회지