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Cytotoxicity Effects of Fraction and Chloroform Extracts from Corn is fructus on Cancer Cell Lines  

Yang Hyun Ok (Department of Cosmetics, Wonkwang Hearth Science College)
Choi Won Hyung (Department of Herbal Rresources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Kim Young Hyun (Division of Natural Science of Natural Sciences College, Professional Graduate School of Oriental Medicine, Wonkwang University)
Baek Seung Hwa (Department of Herbal Rresources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Chun Hyun Ja (Division of Natural Science of Natural Sciences College, Professional Graduate School of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.5, 2004 , pp. 1343-1346 More about this Journal
Abstract
Cornis fructus were extracted by successive extractions and then fractionated with chloroform extract to get active fractions. This study was performed to determine the cytotoxic effect of chloroform extract from Corn is fructus on NIH 3T3 fibroblasts and cancer cell lines using MTT assay. All extracts did not exhibit cytotoxicity in NIH 3T3 fibroblasts. Chloroform extract exhibited antitumor activity in A549, MDA-MB-123, B16 melanoma and SNU-C4 cells. Futher fractionation with chloroform extract was performed to obtain effective fractions. 3 fraction showed the strongest cytotoxic effect against A549, MDA-MB-123, B16 melanoma and SNU-C4 cells. These results suggest that 3 fraction of the chloroform extract from Cornis fructus possessed bioactive material of antitumorous agents.
Keywords
Corni fructus; MTT assay; Cytotoxic effects;
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