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The Effects of Seonghyangjeonggisan on Cytokines Production in the Peripheral Blood Mononuclear Cells of Acute Cerebral Infarction Patients

Yun Jong Min (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University, Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Lee Min Goo (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University, Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Park Sae Wook (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University, Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Lee In (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Cho Kwang Ho (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University, Department of Weurology, College of Pledicine, Wonkwang University)
Moon Byung Soon (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University, Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)

Publication Information

Journal of Physiology & Pathology in Korean Medicine / v.18, no.4, 2004 , pp. 1179-1185 More about this Journal

Abstract

The Korean traditional medicine, Seonghyangjeonggisan (SHJGS) has long been used for acute cerebral infarction (Cl). However, scientific investigation has been carried out a little. Cytokines, involved in the regulation of inflammatory reactions and immune responses, may play an important role in the pathogenesis of Cl. The aim of this study is to investigate the effects of SHJGS on the production of various cytokines in the patients with acute Cl. Peripheral blood mononuclear cells (PBMC) obtained from the patients with acute Cl were cultured for 24hr in the presence or absence of lipopolysaccharide (LPS) and phytohaemagglutinin (PHA). The amount of TNF-α, IL-1β, IL-6 and IL-8, in PBMC culture supernatant, was significantly increased in the LPS and PHA treated cells, compared with unstimulated cells (P<0.05). This study showed that increased TNF-α, IL-1β, IL-6 and IL-8 level stimulated by LPS and PHA was inhibited by SHJGS (0.01-1 ㎎/㎖) in a dose-dependent manner but IL-8 level was not inhibited significantly at 1㎎/㎖ (P>0.05). The maximal inhibition rate of TNF-α, IL-1β, IL-6 and IL-8 by SHJGS (1㎎/㎖) was 68% (P<0.05), 53.9% (P<0.05), 45.5% (P<0.05), 46.7% (P>0.05) respectively. These results suggest that SHJGS might have anti-inflammatory effects through cytokine modulation. which might explain its beneficial effects in the treatment of acute Cl.

Keywords

Seonghyangjeonggisan; acute cerebral infarction; cytokine; peripheral blood mononuclear cells;

Citations & Related Records

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