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Studies on Gene Expression of Imperatorin treated in HL-60 cell line using High-throughput Gene Expression Analysis Techniques  

Kang Bong-Joo (Department of Medical Research, KIOM)
Cha Min-Ho (Department of Medical Research, KIOM)
Jeon Byung Hun (College of Oriental Medicine, Wonkwang University)
Yun Yong Gab (College of Oriental Medicine, Wonkwang University)
Yoon Yoo Sik (Department of Medical Research, KIOM)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.4, 2004 , pp. 1028-1035 More about this Journal
Abstract
Imperatorin, a biologically active furanocoumarin from the roots of Angelica dahurica (Umbelliferae), was mutagenic and induced transformation of mouse fibroblast cell lines, whereas it provided inhibiting effects on mutagenesis and carcinogenesis induced by various carcinogens. Furthermore, it has been suggested that imperatorin may have potential anticarcinogenic effects when administered orally in the diet. In addition to its anticarcinogenic properties, imperatorin has been shown to possess anticancer activities. We investigated the macro scale gene expression analysis on the HL-60 cells treated with imperatorin. Imperatorin (10μM) were used to treat the cells for 6h, 12h, 24h, 48h, and 72h. In a human cDNAchip study of 10,000 genes evaluated 6, 12, 24, 48, 72 hours after treated with imperatorin in HL-60 cells. Hierarchical cluster against the genes which showed expression changes by more than 2 fold. Three hundred eighty six genes were grouped into 6 clusters by a hierarchical clustering algorithm. Pathway analysis using gene microarray pathway prof Her that is a computer application designed to visualize gene expression data on screen representing biological pathways and groupings of genes.
Keywords
imperatorin; microarray; gene expression; pathway analysis; HL-60;
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