[KSCI] Korea Science Citation Index Service

Studies on the Apoptosis-Inducing Effect of Ulmi Pumilae Cortex on Human Leukemia HL-60 Cells

Rhyu Jun Ki (Department of internal medicine, College of Oriental medicine, Wonkwang university)
Yu Bong Seon (Department of internal medicine, College of Oriental medicine, Wonkwang university)
Jeong Jae Eun (Department of internal medicine, College of Oriental medicine, Wonkwang university)
Bak Jin Yeong (Department of internal medicine, College of Oriental medicine, Wonkwang university)
Son In Hwan (Department of internal medicine, College of Oriental medicine, Wonkwang university)
Lee Ju Seok (Department of internal medicine, College of Oriental medicine, Wonkwang university)
Jeon Byeong Hun (Professional Graduate School of Oriental Medicine, Wonkwang university)
Mun Byung Soon (Department of internal medicine, College of Oriental medicine, Wonkwang university)

Publication Information

Journal of Physiology & Pathology in Korean Medicine / v.18, no.3, 2004 , pp. 900-907 More about this Journal

Abstract

The antiproliferative effect of the water extract of the branch and root bark of Ulmi Pumilae Cortex(WEUPC) was investigated on the p53-negative human leukemia cell line (HL-60). A dose- and time-dependent inhibition of cell growth was observed; this effect appears to be due to induction of apoptosis. Involvement of oxidative stress is indicated by a dose-dependent increase in intracellular reactive oxygen species levels. In addition. anti-apoptic effect was observed in the cells simultaneously treated with WEUPC and the anti-oxidant N-acetylcysteine. WEUPC did not affect the anti-apoptotic Bcl-2 and the pro-apoptotic Bax, whereas p21/sup WAF1/CIPl/ was enhanced in a dose- and time-dependent fashion; this effect was partially inhibited by N-acetylcysteine. The increase in p21/sup WAF1/CIPl/ was accompanied by a parallel accumulation of cells in the G1 phase of the cycle. These results suggest that the p53-independent induction of p21/sup WAF1/CIP/ and the induction of apoptosis may mediate the anti proliferative effect of WEUPC at least in this study; on the basis of this observation, WEUPC could be proposed as an useful adjunct to the treatment of p53-deficient tumors, which are often refractory to standard chemotherapy.

Keywords

Ulmi Pumilae Cortex; Human Leukemia HL-60 Cells; Apoptosis;

Citations & Related Records

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