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Inhibition of Platelet Aggregation by Anti-thyroglobulin Monoclonal Antibodies  

Shon Yun Hee (Department of Pharmacology and Intractable Disease Research Center, College of Medicine, Dongguk University)
Kim Cheorl Ho (Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University)
Jeon Byung Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University)
Nam Kyung Soo (Department of Pharmacology and Intractable Disease Research Center, College of Medicine, Dongguk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.2, 2004 , pp. 534-537 More about this Journal
Abstract
We produced twelve monoclonal antibodies(mAbs) against thyroglobulin and characterized the bindig profiles. Among them, three mAbs(TN-1, TN-2 and TN-3) were further characterized their binding specificities. TN-2 had a potent lupus anticoagulant activity and potentiated the anticoagulant effect of venom phospholipase A₂. he anticoagulant mechanism of TN-2 was elongation of the partial thromboplastin time and binding to phosphatidylserine which may have a pivot role in blood coagulation. And TN-2 was cross-reacted with ss-DNA and ds-DNA and had a characteristic of autoantibody. These results suggest that TN-2 may provide a useful tool for studying the correlation between autoimmune thyroiditis and its therapeutic effect.
Keywords
Platelet Aggregation; Anti-thyroglobulin Monoclonal Antibodies;
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1 Effective production of monoclonal antibodies against phosphatidylserine: stereo-specific recognition of phosphatidylserine by monoclonal antibody. /
[ Umeda, M.;Igarashi, K.;Nam, K.S. and Inoue, K. ] / J. Immunol.
2 Autoantibodies in autoimmune thyroid disease promote immune complex formation with self antigens and increase B cell and CD4+ T cell proliferation in response to self antigens. /
[ Nielsen, C.H.;Hegedus, L. and Leslie, R.G. ] / Eur. J. Immunol.   DOI   ScienceOn
3 Autoantibodies in early arthritis: advances in diagnosis and prognostication. /
[ Hueber, W.;Utz. P.J. and Robinson, W,H. ] / Clin. Exp. Rheumatol.
4 Development of autoantibodies before the clinical onset of systemic lupus erythematosus. /
[ Arbuckle, M.R.;McClain, M.T.;Rubertone, M.V.;Scofield, R.H.;Dennis, G.J.;James, J.A. and Harley, J.B. ] / N. Engl. J. Med.   DOI   ScienceOn
5 Structure and function of anti-DNA autoantibodies derived from a single autoimmune mouse. /
[ Shlomchik, M.J.;A.H. Aucoin; D.S. Pisetesky and M.G. Weigert. ] / Proc. Natl. Acad, Sci.   DOI   ScienceOn
6 Antiphospholipid antibodies-autoantibodies with a difference. /
[ Harris, E.N.;Asherson, R.A. and Hughes, G.R.V. ] / Ann. Rev. Med.   DOI   ScienceOn
7 Production and characterization of anti- thyroglobulin monoclonal antibodies /
[ Nam, K.S.;Shon, Y.H.;Baek, S.W.;Kim, C.H.;Lim, J.K. and Hwang, C.W. ] / Korean J. of Life Science
8 The dilure pospholipid APTT: a sensitive assay for verification of lupus anticoagulants. /
[ Alving, B.M.;Baldwin, P.E.;Richards, R.L. and Jackson, B.J. ] / Thromb. Haemostas.
9 Anti-platelet function of the non-saponin fraction from Panax ginseng C.A. Mayer(Korean red ginseng) /
[ Park, H.J.;Rhee, M.H.;Park, K.M.;Nam, K.Y. and Park, K.H. ] / Korean Biochem. J.
10 Human extracellular recombinant phospholipase A2 induces an inflammatory response in rabbit joints. /
[ Bomalaski, J.S.;Lawton, P. amd Browning, J.L. ] / J. Immunol.
11 Effective induction of anti-phospholipid and anticoagulant antibodies in normal mouse. /
[ Igarashi, K.;Umeda, M.;Tokida, S.;Nam, K.S. and Inoue, K. ] / Thrombosis Res.   DOI   ScienceOn
12 Production and characterization of monoclonal antibodies that specifically bind to phosphatidylcholine. /
[ Nam, K.S.;Igarashi, K.;Umeda, M. and Inoue, K. ] / Biochim. Biophys. Acta   DOI
13 Anti-neuronal autoantibody in Hashimoto's encephalopathy: neuropathological, immunohistochemical, and biochemical analysis of two patients. /
[ Oide, T.;Tokuda, T.;Yazaki, M.;Watarai, M.;Mitsuhashi, S.;Kaneko, K.;Hashimoto, T.;Ohara, S. and Ikeda, S. ] / Neurol. Sci.   DOI   ScienceOn