Browse > Article

Down-regulation of COX-2 and hTERT Expression by Healthful Decoction Utilizing Phellinus Linteus in Human Lung Carcinoma Cells  

Park Cheol (Department of Biochemistry, College of Natural Sciences, Dongeui University)
Lee Yong Tae (Department of Physiology, College of Natural Sciences, Dongeui University)
Jeong Young Kee (Department of Microbiology, College of Natural Sciences, Dongeui University)
Choi Byung Tae (Department of Anatomy, Dongeui University College of Oriental Medicine and Research Institute of Oriental Medicine)
Lee Sang Hyeon (Department of Bioscience and Biotechnology, Silla University)
Choi Yung Hyun (Department of Biochemistry, College of Natural Sciences, Dongeui University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.2, 2004 , pp. 500-506 More about this Journal
Abstract
The objective of the present study was to investigate the effects of aqueous extract from the healthful decoction utilizing Phellinus linteus (HDPL) on the growth of human lung carcinoma A549 cells. HDPL treatment declined the cell viability of A549 cells in a concentration-dependent manner and the anti-proliferative effects by HDPL treatment were associated with morphological changes such as membrane shrinking and cell rounding up. HDPL treatment did not affect the distribution of the cell cycle. Western blot analysis and RT-PCT data revealed that the levels of tumor suppressor p53 and cyclin-dependent kinase inhibitor p21WAF1/CIP1 in HDPL-treated A549 cells were remained unchanged. However, HDPL treatment inhibited the expression of cyclooxygenase-2 (COX-2) mRNA and protein in a concentration-dependent fashion. Additionally, the expression of human telomerase reverse transcriptase (hTERT), a main determinant of the telomerase enzymatic activity, was progressively down-regulated by HDPL treatment. Taken together, these findings suggest that HDPL-induced inhibition of human lung cancer cell proliferation is associated with the inhibition of several major growth regulatory gene products, such as COX-2 and hTERT, and HDPL may have therapeutic potential in human lung cancer.
Keywords
HDPL; COX-2; hTERT;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Antitumor activity of some basidiomycetes. especially Phellinus linteus. /
[ Ikekawa, T.; Nakanish, M.; Uehara, N.; Chihara, G.; Fukuoka, E. ] / Gann
2 Agaricus blazei Murill: medicinal and dietry effects. /
[ Mizuno, T.; Kawariharatake ] / Food Rev. Int.   DOI   ScienceOn
3 In vitro and in vivo antitumor activity of the fruit body of Phellinus linteus. /
[ Lee, K.Y.; Han, M.J.; Park, S.Y.; Kim, D.H. ] / Kor. J. Food Sci. Technol.
4 The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. /
[ Harper, J.W.; Adami, G.R.; Wei, N.; Keyomarsi, K.; Elledge, S.J. ] / Cell   DOI   ScienceOn
5 WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis. /
[ El-Deiry, W.S.; Harper, J.W.; O'Connor, P.M.; Velculescu, V.E.; Canman, C.E.; Jackman, J.; Pietenpol, J.A.; Burrell, M.; Hill, D.E.; Wang, Y.; Wiman, K.G.; Mercer, W.E.; Kastan, M.B.; Kohn, K.W.; Elledge, S.J.; Kinzler, K.W.; Vogelstain, B. ] / Cancer Res.
6 Tumor suppressor p53 is a direct transcriptional activator of the human bax gene. /
[ Miyashita, T.; Reed, J.C. ] / Cell   DOI   ScienceOn
7 p21 is a universal inhibitor of cyclin kinases. /
[ Xiong, Y.; Hannon, G.; Zhang, H.; Casso, D.; Kobayashi, R.; Beach, D. ] / Nature   DOI   ScienceOn
8 Multiple pathways to apoptosis. /
[ Evans, V.G. ] / Cell Biol. Int.   DOI   ScienceOn
9 COX-2 inhibition and prevention of cancer. /
[ Giercksky, K.E. ] / Best Pract. Res. Clin. Gastroenterol.   DOI
10 The roles of ERK1/2 and p38 MAP kinases in the preventive mechanisms of mushroom Phellinus linteus against the inhibition of gap junctional intercellular communication by hydrogen peroxide. /
[ Cho, J.H.; Cho, S.D.; Hu, H.; Kim, S.H.; Lee, S.K.; Lee, Y.S.; Kang, K.S. ] / Carcinogenesis   DOI   ScienceOn
11 Study on antitumor and immunomodulatory effects of Cambodian Phellinus linteus /
[ Lee, H.J.; Lee, H.J.; Park, J.M.; Song, G.Y.; Kang, K.S.; Kim, S.H. ] / Kor. J. Oriental Physiol. Pathol.
12 Antimutagenic and cytotoxicity effects of Phellinus linteus extracts. /
[ Ji, J.H.; Kim, M.N.; Chung, C.K.; Ham, S.S. ] / J. Kor. Soc. Food Sci. Nutr.
13 Effects of artificially cultured Phellinus linteus on harmful intestinal bacterial enzymes and rat intestinal <TEX>$\beta$</TEX>-glucocidases. /
[ Kim, D.H.; Choi, H.J.; Bae, E.A. ] / J. Fd. Hyg. Safety
14 Immunostimulating activity of Phellinus linteus extracts to B-lymphocyte. /
[ Oh, G.T.; Han, S.B.; Kim, H.M.; Han, M.W.; Yoo, I.D. ] / Arch. Pharm. Res.
15 Stimulation of hormoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus. /
[ Kim, H.M.; Han, S.B.; Oh, G.T.; Kim, Y.H.; Hong, D.H.; Hong, N.D.; Yoo, I.D. ] / Int. J. Immunopharm.   DOI   ScienceOn
16 Effects of Phellinus linteus extracts on the hormonal immune response in normal and cyclophosphamide-treated mice. /
[ Pyo, M.Y.; Hyun, S.M.; Yang, K.S. ] / J. Applied Pharmacol.
17 Fractionation and antitumor activity of the water-insoluble residue of Agaricus blazei fruiting bodies. /
[ Kawagishi, H.; Inagaki, R.; Kanao, T.; Mizuno, T ] / Carbohydr. Res.
18 Tumoricidal activity of high molecular weight polysaccharides derived from Agaricus blazei via oral administration in the mouse tumor model. /
[ Fujimiya, Y.; Kobori, H.; Oshiman, K.; Soda, R.; Ebina, T. ] / Nippon Shokuhin Kagaku Kogaku Kaishi   DOI
19 In vitro and in vivo antitumor activities of water extracts from Agaricus blazei Murill. /
[ Chun, H.S.; Choi, E.H.; Kim, H.J.; Choi, C.W.; Hwang, S.J. ] / Food Sci. Biotechnol.
20 Antimutagenic and cytotoxicity effects of Agaricus blazei extracts. /
[ Ji, J.H.; Kim, M.N.; Choi, K.P.; Chung, C.K.; Ham, S.S. ] / Kor. J. Food Sci. Technol.
21 The effects of healthful decoction utilizing Phellinus linteus in carbon tetrachloride-injected rats. /
[ Kang, K.H.; Lee, J.H.; Choi, Y.H.; Choi, B.T.; Lee, Y.T. ] / Kor. J. Oriental Physiol. Pathol.
22 Regulation of cyclin D1 by calpain protease. /
[ Choi, Y.H.;Lee, S.J.;Nguyen, P.; Jang, J.S.; Lee, J.; Wu, M.; Takano, E.; Maki, M.; Henkart, P.; Trepel, J.B. ] / J. Biol. Chem.   DOI   ScienceOn
23 Induction of Bax and activation of caspases during <TEX>$\beta$</TEX>-sitosterol-mediated apoptosis in human colon cancer cells. /
[ Choi, Y.H.; Kong, K.R.; Kim, Y.A.; Jung, K.O.; Kil, J.H.; Rhee, S.H.; Park, K.Y. ] / Int. J. Oncol.
24 The retinoblastoma protein and cell cycle control. /
[ Weinberg, R.A. ] / Cell   DOI   ScienceOn
25 Cdk inhibitors: on the threshold of checkpoints and development. /
[ Elledge, S.J.; Harper, J.W. ] / Curr. Opin. Cell Biol.   DOI   ScienceOn
26 Is COX-2 inhibition a panacea for cancer prevention? /
[ Vainio, H. ] / Int. J. Cancer
27 Cyclooxygenase-2: a novel target for cancer chemotherapy? /
[ Dempke, W.; Rie, C.; Grothey, A.; Schmoll, H.J. ] / J. Cancer Res. Clin. Oncol.   DOI   ScienceOn
28 Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-<TEX>$\kappa$</TEX>B activation. /
[ Surh, Y.J.; Chun, K.S.; Cha, H.H.; Han, S.S.; Keum, Y.S.; Park, K.K.; Lee, S.S. ] / Mutat. Res.   DOI   ScienceOn
29 Cyclooxygenase inhibitors suppress angiogenesis and reduce tumor growth in vivo. /
[ Sawaoka, H.; Tsuji, S.; Tsujii, M.; Gunawan, E.S.; Sasaki, Y.;Kawano, S.; Hori, M. ] / Lab. Invest.
30 Therapeutic potential of inhibition of the NF-<TEX>$\kappa$</TEX>B pathway in the treatment of inflammation and cancer. /
[ Yamamoto, Y.; Gaynor, R.B. ] / J. Clin. Invest.   DOI   ScienceOn
31 Activity, function, and gene regulation of the catalytic subunit of telomerase (hTERT) /
[ Poole, J.C.; Andrews, L.G.; Tollefsbol, T.O. ] / Gene   DOI
32 Complex regulatory mechanisms of telomerase activity in normal and cancer cells: How can we apply them for cancer therapy. /
[ Kyo, S.; Inoue, M. ] / Oncogene   DOI   ScienceOn
33 ATM-dependent telomere loss in aging human diploid fibroblasts and DNA damage lead to the post-translational activation of p53 protein involving poly(ADP-ribose) polymerase. /
[ Vaziri, H.; West, M.D.; Allsopp, R.C.; Davison, T.S.; Wu, Y.S.; Arrowsmith, C.H.; Poirier, G.G.; Benchimol, S. ] / EMBO J.   DOI   ScienceOn
34 DNA damage-induced cell cycle checkpoints involve both p53-dependent and -independent pathways: role of telomere repeat binding factor 2. /
[ Narayan, S.; Jaiswal, A.S.; Multani, A.S.; Pathak, S. ] / Br. J. Cancer   DOI   ScienceOn
35 Telomeres, telomerase, and myc. /
[ Cerni, C. ] / An update. Mutat. Res.   DOI   ScienceOn
36 The Pezcoller lecture: cancer cell cycles revisited. /
[ Sherr, C.J. ] / Cancer Res.