[KSCI] Korea Science Citation Index Service

Effects of the Water Extract from Albizzia julibrissin on Apoptosis and Cell Cycle Progression in the Human Leukemic Jurkat Cells

Park Young Jun (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Jung Woo Cheal (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Jeong Dae Young (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Lee Yong Un (Professional Graduate School of Oriental Medicine, Wonkwang University)
Lee In (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Lee Key Sang (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Jeon Byung Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University)
Sung Kang Keyng (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Moon Byung Soon (Professional Graduate School of Oriental Medicine, Wonkwang University)

Publication Information

Journal of Physiology & Pathology in Korean Medicine / v.17, no.6, 2003 , pp. 1383-1392 More about this Journal

Abstract

Apoptosis is a morphologically and biochemically district form of cell death that occurs in many different cell types in a wide variety of organisms. Albizzia julibrissin belonging the family Leguminosae has been used for the treatment of contusion, sore throat, amnesia, and insomnia in oriental traditional medicine. This study investigates whether the water extract of A. julibrissin induce apoptotic cell death in Jurkat T-acute lymphoblastic leukemia (ALL) cells. Jurkat cells were increased inhibitions of cell viability in a concentration-dependent manner by A. julibrissin. This herbal medicine also caused apoptosis as measured by cell morphology and DNA fragmentation. The capability of A. julibrissin to induce apoptosis was associated with proteolytic cleavage of specific target proteins such as poly (ADP-ribose)polymerase (PARP) and beta-catenin proteins suggesting the possible involvement of caspases. Our result showed that Bcl-2 and Bax protein levels were not changed in all A. julibrissin-treated groups compared to control group. These results suggest that A. julibrissin-mediated apoptosis is independent with Bcl-2 related signaling pathway in this cells. The purpose of the present study is also to investigate the Effect of A. julibrissin on cell cycle progression. Our results showed that G1 checkpoint related gene products (cyclin D1, cyclin dependent kinase 4, retinoblastoma, E2F1) were decreased in their protein levels in a dose-dependent manners after treatment of the extract. These results indicate that the increase of apoptotic cell death by A. julibrissin may be due to the inhibition of cell cycle progression in wild type p53-lacking Jurkat cells.

Keywords

Albizzia julibrissin; Apoptosis; Cell Cycle Progression; Human Leukemic Jurkat Cells;

Citations & Related Records

Reference

1	Sedative activity of two flavonol glycosides isolated from the flowers of Albizzia julibrissin Durazz / [ Kang, T.H.;Jeong, S.J.;Kim, N.Y.;Higuchi, R.;Kim, Y.C. ] / J. Ethnopharmacol. ScienceOn
2	Formation of high molecular mass DNA fragments is a marker of apoptosis in the human leukaemic cell line. U937. / [ Bicknell, G.R.;Snowden, R.T.;Cohen, G.M. ] / J. Cell Sci.
3	Proteolysis of fodrin (non-erythroid spectrin) during apoptosis / [ Martin, S.J.;OBrien, G.A.;Nishioka, W.K.;McGahon, A.J.;Sadio, T.;Green, D.R. ] / J. Biol. Chem. ScienceOn
4	Human ICE/CED-3 protease nomenclature / [ Alnemri, E.S.;Livingston, D.J.;Nicholson, D.W.;Salvesen, G.;Thornberry, N.A.;Wong, W.W.;Yuan, J. ] / Cell ScienceOn
5	Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis / [ Kaufmann, S.H.;Desnoyers, S.;Ottaviano, Y.;Davidson, N.E.;Poirier, G.G. ] / Cancer Res.
6	Cleavage of retinoblastoma protein during apoptosis: an interleukin 1 beta-converting enzyme-like protease as candidate / [ An, B.;Dou, Q.P. ] / Cancer Res.
7	Proteolysis of beta-catenin in apoptotic Jurkat cells / [ Hwang, S.G.;Park, J.U.;Lee, H.C.;Joo, W.H.;Cho, Y.K.;Moon, J.Y. ] / J. Life Sci.
8	The p53 network / [ Agarwal, M.L.;Taylor, W.R.;Chernov, M.V.;Chernova, O.B.;Stark, G.R. ] / J. Biol. Chem. ScienceOn
9	Cyclins and cancer II: cyclin D and CDK inhibitors come of age / [ Hunter, T.;Pines, J. ] / Cell ScienceOn
10	The retinoblastoma protein and cell cycle control / [ Weinberg, R.A. ] / Cell ScienceOn
11	/ [ 具本泓(外) ] / 東醫內科學
12	/ [ 陣貴廷(外) ] / 實用中西醫結合診斷治療學
13	/ [ 문구;정병학;김병주 ] / 癌東西醫結合治療
14	Toward an understanding of the molecular mechanisms of physiological cell death / [ Vaux, D.L. ] / Proc. Natl. Acad. Sci. ScienceOn
15	Apoptosis and the regulation of cell numbers in normal and neoplastic tissues: an overview / [ Wyllie, A.H. ] / Cancer Meta. Rev. ScienceOn
16	Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation / [ Gavrieli, Y.;Sherman, Y.;Ben-Sasson, S.A.J. ] / J. Cell Biol. ScienceOn
17	Bcl-2 gene promotes hemopoietic cell survival and cooperates with c-myc to immortalize pre-B cells / [ Vaux, D.L.;Cory, S.;Adams, J. ] / Nature ScienceOn
18	Inhibitory Effects of extracts from traditional herbal drugs on 5-Hydroxytryptophan- induced diarrhea in mice / [ Yoo, J.S.;Jung, J.S.;Lee, T.H.;Son, K.H.;Suh, H.W.;Song, D.K.;Kim, Y.H. ] / Kor. J. Pharmacogn.
19	/ [ 金昌玟(外) ] / 完譯中葯大辭典
20	/ [ 高學敏(編) ] / 中藥學(下冊)
21	/ [ 申佶求 ] / 申氏本草學(各論)
22	/ [ 鄭普燮(外) ] / 圖解鄕藥大事典(植物篇)
23	/ [ 吳普(述著) ] / 神農本草經(卷二)
24	/ [ 上海中醫學院 ] / 中草藥學
25	/ [ 李時珍 ] / 本草綱目(交點本)
26	/ [ 謝觀 ] / 中國醫學大辭典
27	/ [ Tang, W.;Eisenbrand, G. ] / Chinese drugs of plant origin
28	How cells cycle toward cancer / [ Marx, J. ] / Science
29	Stifled by inhibitions / [ Gordon, P. ] / Nature ScienceOn
30	/ [ 郁仁存 ] / 中醫腫瘤學
31	/ [ 李岩 ] / 腫瘤臨證備要
32	/ [ 李岩 ] / 腫瘤學
33	/ [ 方藥中 ] / 實用中醫內科學
34	/ [ 張代釗 ] / 中西醫結合治療癌症
35	韓藥을 利用한 抗癌實驗硏究의 傾向에 關한 考察 / [ 김현아;임성우;이철원 ] / 大韓韓方腫瘍學會誌
36	A Review of research on plants for fertility regulation in Korea / [ Woo, W.S.;Lee, E.B.;Shin, K.H. ] / Kor. J. Pharmacogn.
37	Effects of antitumor polysaccharides from Albizza julibrissin on immune function / [ Moon, C.K.;Lee, B.G.;Lee, S.H. ] / Arch. Pharm. Res.
38	Screening of domestic plants with antibacterial activity / [ Yang, M.S.;Ha, Y.L.;Nam, S.H.;Choi, S.U.;Jang, D.S. ] / Agricultural Chemistry and Biotechnology
39	Albizzia julibrissin Durazz 엑기스의 抗眞菌作用 / [ 李京淳(外) ] / 忠北大藥學論文集
40	An arrhythmic-inducing glycoside from Albizzia julibrissin Durazz. IV. / [ Higuchi, H.;Kinjo, J.;Nohara, T. ] / Chem. Pharm. Bull
41	Specific cleavage of the 70-kDa protein component of the U1 small nuclear ribonucleoprotein is a characteristic biochemical feature of apoptotic cell death / [ Casciola-Rosen, L.A.;Miller, D.K.;Anhat, G.J.;Rosen, A. ] / J. Biol. Chem.
42	/ [ 圓光大學校第21期(編譯) ] / 本草求眞
43	/ [ 錢伯文 ] / 腫瘤的辨證施治

KSCI

Effects of the Water Extract from Albizzia julibrissin on Apoptosis and Cell Cycle Progression in the Human Leukemic Jurkat Cells 백혈병 세포주 Jurkat의 세포고사 유도 및 세포주기 억제에 미치는 합산피 추출물의 효과

Effects of the Water Extract from Albizzia julibrissin on Apoptosis and Cell Cycle Progression in the Human Leukemic Jurkat Cells