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Induction of S phase Arrest of the Cell Cycle by Oak Smoke Flavoring (Holyessing) in Human Prostate Carcinoma Cells  

Park Cheol (Department of Biochemistry. College of Oriental Medicine, Dongeui University)
Lee Won Ho (Department of Biology. College of Natural Sciences, Busan National University)
Choi Byung Tae (Department of Anatomy, Dongeui University)
Kim Kyoung Chul (Department of Physiology, College of Oriental Medicine, Dongeui University and Research Institute of Oriental Medicine)
Lee Yong Tae (Department of Physiology, College of Oriental Medicine, Dongeui University and Research Institute of Oriental Medicine)
Choi Yung Hyun (Department of Biochemistry. College of Oriental Medicine, Dongeui University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.17, no.5, 2003 , pp. 1309-1314 More about this Journal
Abstract
We examined the effects of Oak Smoke Flavoring (OSF, Holyessing) on the cell proliferation of DU145 and PC3 human prostate carcinoma cell line. OSF treatment resulted in a concentration-dependent inhibition of the cell viability in both DU145 and PC3 cell lines. The anti-proliferative effects by OSF treatment in DU145 and PC3 cells were associated with morphological changes such as membrane shrinking and cell rounding up. DNA flow cytometric histograms showed that population of S phase of the cell cycle was increased by OSF treatment in a dose-dependent manner. Western blot analysis revealed that cyclin B1 and cdc2 proteins were reduced by OSF treatment in DU145 cells, whereas cyclin A was markedly inhibited in PC3 cells. Furthermore, we observed an increase of Cdk inhibitor p16 and p27 protein, and an inhibition of phosphorylation of pRB by OSF treatment in a dose-dependent manner. The present results indicated that OSF-induced inhibition of human prostate carcinoma cell proliferation is associated with the blockage of S phase progression.
Keywords
Oak Smoke Flavoring; prostate cancer cells; S arrest, cyclin; cdk;
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