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Cytotoxic Activity of Methanol Fractions and Solvent Extracts from Houttuynia cordata $T_{HUNS}$ (IX) on Various Cancer Cells  

Lee Jeong Ho (Department of Third Medicine, Wonkwang University)
Baek Seung Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Lim Jin A (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
Chun Hyun Ja (Division of Natural Science, Wonkwang University)
Lee Ki Nam (Department of Third Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.17, no.5, 2003 , pp. 1288-1292 More about this Journal
Abstract
This study was carried out to evaluate cytotoxic effects of Houttuynia cordata T/sub HUNB/ extracts on A549 (lung cancer), MDA-MB231 (breast cancer), SNU-C4 (colon cancer) and B16 (mouse melanoma) cell lines. We have determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay. The 150 ㎍/㎖ concentration of methanol extract (63.81 %) of Houttuynia cordata T/sub HUNB/ was shown significantly antitoxic activity on A549 cell lines. The order of cytotoxicity fractions of methanol from Houttuynia cordata T/sub HUNB/ extracts against cancer cell lines in vitro is as follows : hexane fraction layer > chloroform fraction layer > ethyl acetate fraction layer > buthanol fraction layer > water fraction layer. These results suggest that the hexane fraction of methanol extract from Houttuynia cordata T/sub HUNB/ extract may be a valuable choice for the development of antitumor agents.
Keywords
Houttuynia cordata T/sub HUNB/; MTT assay; Cytotoxic effect;
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Times Cited By KSCI : 4  (Citation Analysis)
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