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Effect of Oxidative Stress and Glutamate Receptor Antagonist on Cultured Rat Osteoblast and Osteoclast  

Park Seung Taeck (School of Medicine, Institution of Wonkwang Science, Wonkwang University)
Jeon Seung Ho (School of Medicine Wonkwang University)
Lee Byung Chan (School of Medicine Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.17, no.4, 2003 , pp. 996-1001 More about this Journal
Abstract
It is well known that oxidative stress of reactive oxygen species(ROS) may be a causative factor in the pathogenesis of bone disorder. The purpose of this study was to evaluate the cytotoxicity of oxidative stress. Cell viability by MTS assay or INT assay, activity of glutathione peroxidase(GPx), lipid peroxidation(LPO) activity and cell viablity. And also protctive effect of glutamate receptors against ROS-induced osteotoxicity was examined by protein synthesis, alkaline phosphatase (ALP) activity and lactate dehydrogenase (LDH) activity in cultured rat osteoblasts and osteoclasts. XO/HX decreased cell viability and GPx activity, protein synthesis and ALP activity, but increased LPO activity and LDH activity. In the protective effect, N-methyl-D-aspartate (NMDA) receptor antagonists or AMPA/kainate receptor antagonists such as D-2-amino-5-phosphonovaleric acid (APV), 7-chlorokynurenic acid (CKA), 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and 6,7-dinitroquinoxaline-2,3-dione (DNQX), NMDA receptor antagonists but AMPA/kainate receptor antagonists showed protective effect on xanthine oxidase (XO) and hypoxanthine (HX) in these cultures by the increse of protein synthesis, ALP activity.
Keywords
Osteoblast; Osteoclast; Glutamate receptor;
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1 Osteoclast-like cell formation and its regulation by osteotropic hormones in mouse bone marrow cultures. /
[ Takahashi N;Suda T ] / Endocrinlogy   DOI   ScienceOn
2 Effect of Ginseng Radix Against Osteoblasts Damaged by Endocrine Disrupting Chemicals. /
[ Hong GY;Park ST;Jeon BH;Seo EA ] / Korea J Oriental Physicol&Pathol.
3 The impact of diabetes on the CNS. /
[ McCall AL ] / Diabetes   DOI   ScienceOn
4 Rapid colorimetric assay for the cellular growth and survival:Application to proliferation and cytotoxic assays. /
[ Mosmann T ] / J Immun Methods   DOI
5 Excitatory amino acid and free radical formation may cooperate in the genesis of ischemia-induced meuronal damage. /
[ Pellegrini-giampietro DE;Cherici G;Alesiani M;Carrla V;Moroni F ] / J Neurosci   DOI
6 Comparisons of two in vitro cytotoxicity assays-the neutral red and tetrazolium MTT tests. /
[ Borenfreund E;Babich H;Martin-Alguacil N ] / Toxic in Vitro   DOI   ScienceOn
7 Study on the effect of iron-chletor on oxygen radical-induced neurotoxicity. /
[ Park ST ] / Korean J Phys Anthrop   DOI
8 오배자 황산화 활성성분과 자유라디칼 소거효과 /
[ 차배천;이승배;임태진;이광희 ] / 생약학회지
9 MNDA receptors activate the arachidonic acid cascade system in striatal neurons. /
[ Dumuis A;Sebben M;Haynes L;Pin J-P;Bockaert J ] / Nature (London)   DOI   ScienceOn
10 Appearance of superoxide anion radical in cerebral extracellular space during increased prostaglandin synthesis in cat. /
[ Kontos H;Wei E;Ellis E Jenkins L;Povlishock J;Rowe G;Hess M ] / Circ Res   DOI
11 Hyperglycemic can cause membrane lipid peroxidation and osmotic fragility in guman red blood cells. /
[ Jain SK ] / J.Biolo Chem
12 Reactive oxygen species and the central nervous system. /
[ Halliwell B ] / J Neurochem   DOI
13 The role of increased oxidative stress in the development of diabetic nephropathy. /
[ Jang YJ;Park H;Kim HS;Hong HN;Kim MK ] / Korean J Pharmacology
14 Inhibition of oxidation of methy1 linoleate in solution by vitamin E and vitamin C. /
[ Niki E;Saito T;Kawakami A;Kamiya Y ] / J.Biol Chem
15 Determination of superoxide dismutase activity by the polarographic method of catalytic currents in the cerebrospinal fluid of aging brain and neurologic degenerative diseases. /
[ Bracco F;Scarpa M;Rigo A;Battistin L ] / Proc Soc Exp Biol Med
16 Oligodendroglial cell death induced by oxygen radicals and its protection by catalase. /
[ Kim YS;kim SU ] / J Neurosci Res   DOI   ScienceOn
17 Antioxidant treatment prevents the development of peripheral nerve dysfunction in streptozotocin-diabetic rats. /
[ Camerone NE;Cotter MA;maxfield EK ] / Diabetologia   DOI   ScienceOn