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Effect of Radix Polygoni Multiflori on Spinal Motor Neurons Damaged by Methylmercuric Chloride  

Ha Dae Ho (School of Medicine Wonkwang University)
Lee Byung Chan (School of Medicine Wonkwang University)
Lee Kang Chang (Department of Graduate School of Oriental Medicine, Wonkwang University)
Lee Whan Bong (School of Medicine Wonkwang University, Institute of Wonkwang Science)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.17, no.3, 2003 , pp. 738-741 More about this Journal
Abstract
In order to evaluate the cytotoxicity of methylmercuric chloride(MMC) in cultured spinal motor neurons of neonatal mouse, cell viability was measured by MTT assay in spinal motor neurons treated with 1-30 μM MMC for 48 hours. And also, the protective effect of Radix Polygoni Multiflori(RPM) was examined by cell viability in these cultures. Cell viability was significantly decreased in dose-dependent manner after cultured cells were exposured to 20 μM MMC for 48 hours. Protective effect of RPM on MMC-mediated toxicity was very effective in these cultures. From above the results, it suggests that MMC has toxic effect in cultured mouse spinal motor neurons and herb extract such as RPM is very effective in blocking the neurotoxicity induced by MMC.
Keywords
Radix Polygoni Multiflori; Cultured spinal motor neuron; Methylmercuric chloride;
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1 Methylmercury-induced neurotoxicity in cerebral neuron culture is blocked by antioxidants and NMDA receptor antagonists. /
[ Park ST;Lim KT;Chung YT;Kim SU ] / Neurotoxicol
2 Scavenger of active oxygens by melanoidin. /
[ Hayase F;Hirashima S;Okamoto G;Kato H ] / Agric Biol Chem
3 Excitatory amino acid and free radical formation may cooperate in the genesis of ischemia-induced neuronal damage /
[ Pellegrini-Giampietro DE;Cherici G;Alesiani M;Carla V;Moroni F ] / J Neurosci
4 Oxygen radical-induced neurotoxicity in spinal cord neuron cultures. /
[ Michikawa M;Lim KT;McLarnon JG;Kim SU ] / J Neurosci Res
5 Rapid colorimetric assays for cellular growth and survival: application to proliferation and cytotoxicity assays. /
[ Mosmann, T. ] / J. Immunol. Methods
6 A simple quantitative procedure using monolayer culture for cytotoxicity assay (HTD/ NR-90). /
[ Borenfreund E.;Puerner J. A. ] / J Tiss Cult Meth
7 A study on the cytotoxicity of cadmium in vitro. /
[ Chung YT;Park ST;Choi MK;Kim JJ;Mun YJ;Woo WH;Han DS;Choi BK;So JT ] / Korean J Toxicol
8 Cadmium -induced DNA strand damage in cultured liver cells : reduction in cadmium genotoxicity following zinc pretreatment. /
[ Coogan TP;Bare RM;waalkes MP ] / Toxicol Appl Pharmacol
9 Effects of inorganic lead on the differentiation and growth of cultured hippocampal and neuroblastoma cells. /
[ Audesirk T;Audesirk G;ferguson C;Shugarts D ] / Neurotoxicol
10 The effect of vitamin E on the toxicity of alkyl mercurials on nervous tissue in culture. /
[ Kasuya M ] / Toxicol Appl Pharmacol
11 Methylmercury toxicity in the chick embryo. /
[ Greener, Y.;Kochen J.A. ] / Teratology
12 Growth factors proven mitochondrial dysfunction, loss of calcium homeostasis and cell injury, but not ATP depletion in hippocampal neurons. /
[ Mattson MP;Zhang Y;Bose S ] / Exp Neurol
13 /
[ Friberg L;Kjellstrom, T;Bronner F, Coburun JW(eds) ] / Cadmium : In "Disorders of Mineral Metabolism".
14 /
[ Tsubaki T;Takahashi H ] / Recent advances in Minamata disease studies.
15 Effect of Antioxidants on FeSO₄Toxicity in Cultured Myocardial Cells. /
[ Park OK;Oh JM;Choi MK;Park ST;Chung YT ] / Korean J Physical Anthropolo
16 Interactions of vitamin E and selenium with mercury and silver. /
[ Ganther HE ] / Ann NY Acad Sci