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Induction of Apoptotic Cell Death by Insamsapye-tang Extract in Human Lung Cancer A549 Cells  

Park Cheol (Department of Biochemistry, and Internal Medicine, College of Oriental Medicine, Dongeui University)
Lee Min Woo (Research Institute of Oriental Medicine, College of Oriental Medicine, Dongeui University)
Kim Won Il (Research Institute of Oriental Medicine, College of Oriental Medicine, Dongeui University)
Lee Won Ho (Department of Biology, College of Natural Sciences, Busan National University)
Park Dong Il (Research Institute of Oriental Medicine, College of Oriental Medicine, Dongeui University)
Choi Yung Hyun (Department of Biochemistry, and Internal Medicine, College of Oriental Medicine, Dongeui University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.17, no.3, 2003 , pp. 677-683 More about this Journal
Abstract
We investigated the effects of Insamsapye-tang (ISSPT) water extract on the growth of human lung carcinoma A549 cells. Upon treatment with ISSPT extract, a concentration-dependent inhibition of cell viability was observed and cells developed many of the hallmark features of apoptosis, including condensation of chromatin. Flow cytometry analysis confirmed that ISSPT treatment increased populations of apoptotic-sub G1 phase. In addition, proteolytic degradation of poly(ADP-ribose) polymerase (PARP) and β-catenin protein were observed after treatment of ISSPT extract. These apoptotic effects of ISSPT in A549 cells were associated with marked inhibition of Bel-xL expression in a dose-dependent manner, however the levels of Bcl-2 and Bax expression were not affected. ISSPT treatment also induced the expression of tumor suppressor p53 mRNA and inhibited the expression of caspase-3 mRNA. The previous and present results indicated that ISSPT-induced inhibition of lung cancer cell proliferation is associated with the blockage of G1/S progression and the induction of apoptosis.
Keywords
Insamsapye-tang(人蔘瀉肺湯); lung carcinoma; apoptosis; PARP; β-catenin; Bel-xL;
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