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Induction of Apoptotic Cell Death by Sabaek-san Extract in Human Lung Cancer A549 Cells  

Lee Jae Hun (Department of Biochemistry, College of Oriental Medicine, Dongeui University)
Kang Byong Ryeung (Department of Internal Medicine, College of Oriental Medicine, Dongeui University and Research Institute of Oriental Medicine)
Kam Cheol Woo (Department of Internal Medicine, College of Oriental Medicine, Dongeui University and Research Institute of Oriental Medicine)
Park Dong Il (Department of Internal Medicine, College of Oriental Medicine, Dongeui University and Research Institute of Oriental Medicine)
Choi Yung Hyun (Department of Biochemistry, College of Oriental Medicine, Dongeui University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.17, no.2, 2003 , pp. 451-456 More about this Journal
Abstract
We investigated the effects of Sabaek-san (SBS) water extract on the growth of human lung carcinoma A549 cells. Upon treatment with SBS extract, a concentration-dependent inhibition of cell viability was observed and cells developed many of the hallmark features of apoptosis. including condensation of chromatin. Flow cytometry analysis confirmed that SBS treatment increased populations of apoptotic-sub G1 phase. In addition. proteolytic cleavages of poly(ADP-ribose) polymerase and β-catenin protein were observed after treatment of SBS extract. These apoptotic effects of SBS in A549 cells were associated with marked inhibition of Bcl-2 and Bel-xL mRNA in a dose-dependent manner. however the levels of Bax expression were not affected, SBS treatment also induced a proteolytic activation of caspase-3. which is believed to play a central role In the apoptotic signaling pathway. The previous and present results indicated that SBS-induced inhibition of lung cancer cell proliferation is associated with the blockage of G1/S progression and the induction of apoptosis.
Keywords
Sabaek-san(瀉白散); lung carcinoma; apoptosis; Bax; Bcl-2; caspase-3;
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