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Study on the Effect of Vitamin E on Cultured Hippocampal Neurons Damaged by Hydrogen Peroxide  

Lee Jung Hun (School of Medicine Wonkang University)
Lee Joung Hwa (School of Medicine Wonkang University)
Cho Nam Su (Department Emergency, School of Medicine Chosun University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.17, no.2, 2003 , pp. 447-450 More about this Journal
Abstract
To clerify the cytotoxicity of reactive oxygen species in cultured hippocampal neurons of neonatal mouse, toxic effect was measured by MTT assay after cultured cells were incubated for 3 hours in the media containing 1～40 μM concentrations of H₂O₂. In addition, the protective effect of vitamin E was determined in these cultrures. Cell viability was significantly decreased in a dose-dependent manner after exposure of 10 μM H₂O₂ to cultured mouse hippocampal neurons for 5 hours. In the protective effect of vitamin E, vitamin E prevented the H₂O₂-induced cytotoxicity in these cultures. From these results, it suggests that H₂O₂ has toxic effect in cultured mouse hippocampal neurons and vitamin E has protective effect on the cytotoxicity induced by H₂O₂.
Keywords
Reactive oxygen species; Vitamin E; Cultured hippocampal neuron;
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