[KSCI] Korea Science Citation Index Service

Effects of Jingansikpung-tang and Gamijingansikpung-tang Water Extract on the Cultured Spinal Sensory Neurons

Seo Young Suk (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University)
Yun Sang Hak (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University)
Yeom Seung Ryong (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University)
Lee Su kyung (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University)
Shin Byung Cheul (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University)
Kwon Young Dal (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University)
Song Yung Sun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University)

Publication Information

Journal of Physiology & Pathology in Korean Medicine / v.17, no.2, 2003 , pp. 374-379 More about this Journal

Abstract

To evaluate the mechanism of oxidative damage by Xanthine oxidase(XO) and hypoxanthine(HX)-induced oxygen radicals, XTT assay was carried out. Neurofilament EIA and PKC activity were measured to evaluate the protective effect of Jingansikpung-tang(JST) and Gamijingansikpung-tang(GJST) water extract on cultured spinal sensory neurons damaged by XO/HX, after the cultured mouse spinal sensory neurons were preincubated with various concentrations of JST and GJST water extract for 3 hours prior to exposure of XO/HX. The results were XO/HX decreased significantly, in proportion to concentration and exposed time, the survival rate of the cultured mouse sensory neurons on XTT assay. And in proportion to concentration and exposed time on cultured spinal sensory neurons, XO/HX showed the quantitative decrease of neurofilament by EIA, increase of PKC activity, but JST and GJST showed the neuroprotective effects against decrease of neurofilament and increase of PKC activity by XO/HX. From the above results, it is concluded that XO/HX have a neurotoxic effect on cultured spinal sensory neurons and the herbs water extract, such as JST and GJST prevent the toxicity of XO/HX effectively.

Keywords

Jingansikpung-tang(鎭肝熄風湯); Gamijingansikpung-tang(加味鎭肝熄風湯); XTT; Neurofilament; XanthineOxidase(XO); Hypoxanthine(HX); PKC activity;

Citations & Related Records

Reference

1	太陰調胃湯이 Glucose oxidase에 의해 손상된 대뇌피질신경세포에 미치는 영향 / [ 김종관 ] /
2	太陰人淸心蓮子湯이 Hydrogen Peroxidase에 손상된 白鼠의 대뇌신경세포에 미치는 영향 / [ 옥윤영 ] /
3	鎭肝熄風湯濫液이 GO에 의해 손상된 培養脊髓運動神經細胞에 미치는 영향 / [ 박광수 ] /
4	加味鎭肝熄風湯이 손상된 培養脊髓運動神經細胞에 미치는 영향 / [ 김성환 ] /
5	痺證의 形證과 病域에 관한 문헌적 고찰 / [ 송봉근 ] /
6	/ [ 전국한의과대학재활의학과학교실(편) ] / 동의재활의학과학
7	/ [ 한국한의학연구원 ] / 한의진단명과 진단요건의 표준화연구(III)
8	/ [ 張錫純 ] / 醫學衷中參書錄
9	/ [ 최호석 ] / 한방임상입문
10	동의보감에 나타난 麻木不仁에 대한 小考 / [ 조한숙;송래원;김신석 ] / 한방재활의학과학회지
11	麻木에 관한 문헌적 고찰 / [ 정석희;이종수;김성수;신현대 ] / 대한한의학회지
12	/ [ 김상찬(외) ] / 方齊學
13	/ [ 이상인;박선동 ] / 韓方臨床處方學
14	/ [ 강소신의학원(편) ] / 중약대사전
15	허혈 유도에 의해 손상된 신생쥐의 배양 대뇌신경세포에 대한 vitamin E와 Desferrioxamine의 보호효과 / [ 이소라 ] /
16	Increased endothelial albumin permeability mediated by protein kinase C activation. / [ Lynch, J.J;ferro, T.J.;Blumenstock, F. A.;Brockenauer, A.M.;Malik, A. M. ] / J Clin Invest DOI
17	70-kDa heat shock protein and c-fos gene expression after transient ischemia. / [ Nowak, T.S.;Ikeda, J.;Nakajima, T. ] / Stroke
18	Excitatory amino acid release from rat hippocampal slices as a consequence of free radical formation. / [ Pellegrini-Ciampietro, D.E.;Cherici, C.;Alesiani, M.;Carrla,V.;Moroni, F. ] / J Neurochem DOI
19	Intracellular signaling by hydrolysis of phospholipids and activation of protein kinase C. / [ Nishizuka, Y. ] / Science DOI
20	Mutation in Cu/zn superoxide dismutase gene are associated with familial amyotrophic lateral sclerosis. / [ Rosen, D. R.;Siddique, T.;Patterson, D.;Figlewicz, D. A.;Sapp, p.;Hentati, A.;Donaldson, D.; Goto, J.;O'Regan J.P.;Deng H.X;Rahmani Z.;Krrizus A. ] / Nature(London) DOI
21	/ [ 김명호 ] / 기초임상신경학
22	/ [ 김창환;김용석 ] / 마비질환클리닉
23	/ [ 대한신경외과학회 ] / 신경외과학
24	/ [ 이광우;정희원 ] / 임상신경학
25	/ [ 피터두스 ] / 신경국소진단학
26	The nerve growth factor Thirty-five tears later. / [ Levi-Montalcin. ] / JEMBO(Eur Mol Biol Organ)
27	Receptors for insulin-like growth factors I and II Autoradiographic localization in rat brain and comparison to receptors for insulin. / [ Lesniak, M.A.;Hill, J.M.;Kiess, W.;Rojeski, M.;Pert, C.B.;Roth, J. ] / Endocrinology DOI
28	/ [ 김완희;최달영 ] / 臟腑辨證論治
29	/ [ 陣貴廷;楊思澍 ] / 賣用中西醫結合治療
30	Oxygen radical-induced neurotoxicity in spinal cord neuron cultures. / [ Michikawa, M.;Lim, K.T.;McLamon, J.G.;Kim, S.U. ] / J Neurosci Res DOI
31	Modulation of the insulin factor II/mannose6-phosphate receptor in microvasrular endothelial cells by phobol ester via protein kinase C. / [ Hu, K.-Q.;Backer, J.M.;sahagian, G.;Feener, E.P.;King, C.L. ] / J Biol Chem
32	/ [ 이원택;박경아 ] / 의학신경해부학
33	/ [ 아담스신경과학편찬위원회(편) ] / 신경과학
34	加味十全大補湯湯液이 Xanthulr oxidase/Xanthi에 의해 손상된 培養脊髓運動神經細胞에 미치는 영향 / [ 이영보;송용선 ] / 한방재활의학과학회지

KSCI

Effects of Jingansikpung-tang and Gamijingansikpung-tang Water Extract on the Cultured Spinal Sensory Neurons 진간식풍탕 및 가미진간식풍탕 추출물이 배양 척수감각신경세포에 미치는 영향

Effects of Jingansikpung-tang and Gamijingansikpung-tang Water Extract on the Cultured Spinal Sensory Neurons