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Effect of Salviae Multiorrhizae Radix on the Vasculotoxicity induced by glucose oxidase in cultured Pumonary Endotherial cells  

Bak Sang Myeon (School of Medicine, , Wonkwang University)
Lee Joung Hwa (School of Medicine, , Wonkwang University)
Yang Hyun Woong (School of Medicine, , Wonkwang University)
Lee Kang Chang (Graduate School of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.17, no.1, 2003 , pp. 136-139 More about this Journal
Abstract
Cytotoxicity of glucose oxidase(GO) and cardioprotective effect of Salviae Multiorrhizae Radix(SMR) against GO-induced cardiotoxicity were measured for evaluation of cardiotoxicity on cultured mouse pulmonary endotherial cells(PEC) by MTT assay after PEC were cultured for 8 hours at various concentrations of GO. GO was toxic in a time and dose-dependent manner on cultured PEC after PEC were grown for 8 hours in media containing 1～60mU/ml GO. While, cultures were pretreated with 60 μg/ml SMR for 2 hours increased remarkably cell viability. From the above results, it is suggested that GO is toxic on cultured PEC by the decrease of cell viability, and herb medicine such as SMR is very effective in the prevention of vascular toxicity induced by GO.
Keywords
Glucose oxidase; Cultured pulmonary endotherial cell; Salviae Multiorrhizae Radix;
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1 Neuroprotective effects of early vs. late administration of dantrolene in experimental status epilepticus. /
[ Marivi N.;Michael G ] / Neuropharmacology   DOI
2 Hydrogen peroxide and hydroxyl radical mediation of activated leukocyte depression og cardiac sarcoplasmic reticulum Participation of cyclooxygenase pathway. /
[ Row GT;Manson NH;Caplan M;Hess MC ] / Cir Res   DOI
3 Vitamin E and lipid antioxidants in free¬radical inhibited reactions. /
[ Witting LA;pryor W A,(ed) ] / Free Radicals in Biology
4 Role of oxygen free radicals in carcinogenesis and brain ischemia. /
[ Floyd RA ] / FASEB J   DOI
5 Conjugated dienes in ischemic and reperfused myocardium free radical mediated injury. /
[ Romaschin A. D.;Rebeyka I.;Wilson G. J.;Mickle D.A.G. ] / J M Cell Cardiol   DOI
6 Intracellular calcium transients in cardiac myocytes exposed to carbon tetrachlo-ride(Abstract). /
[ Toraason M;Breitenstein M ] / Toxicologist
7 Superoxide dimutase plus catalase improve contractile function in the canine model of "stunned myocardium" . /
[ Przyklenk K;Kloner RA ] / Cir Res
8 사군자탕 및 사물탕이 methotrexate로 유도된 흰쥐의 면역기능저하에 미치는 영향. /
[ 최윤정;김장현 ] / 대한한방소아과학회지
9 A possible role of lipid peroxidation in cellular damages caused by cerebral ischemia and protective effect of alpa-tocopherol administration. /
[ Yamamoto M;Shima T;Uozumi T; Sogabe T;Yamada K;Kawasaki T ] / Stroke   DOI   ScienceOn
10 Excitatory amino acid release from rat hippocampal slices as a consequence of free radical formation. /
[ Pellegrini-Giampietro D. E.;Cherici G.;Alesiani M.;Carla V.;Moroni F ] / J Neurochem   DOI
11 Glutamate receptors in the substentia nigra of Parkinson's disease brains. /
[ Difazio M, C.; Hollingsworth Z.;Young A. B .;Penny J.B. ] / Neurology.   DOI
12 Cytology and cytochemistry of mammalian myocardial cells in culture. /
[ Kasten FH ] / Acta Histochem
13 육미지황탕이 면역세포에 미치는 실험적 효과 /
[ 전진오;정현우 ] / 대한한방내과학회지
14 Mechanism for depression of heart sarcolemmal ca<TEX>$^{2+}$</TEX> pump by oxygen free radicals. /
[ Kaneko M;Elmban V;Dhalla NS ] / Am J Physiol