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The Screening of Hepatic Functional Improvement, Liver Protection and Antifibrotic Effect from Dried Extracts of Concha Cipangopaludinae in Rats  

Kim Hee Seok (Professional Graduate School of Oriental Medicine, Medical School Wonkwang University)
Kim Jin Sook (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine)
Kim Ki young (Professional Graduate School of Oriental Medicine, Medical School Wonkwang University, Department of Pathology, Medical School Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.17, no.1, 2003 , pp. 118-122 More about this Journal
Abstract
Oxidative stress and its consequent lipid peroxidation exert harmful effects, which have been currently involved in the generation of carbon tetrachloride (CCl₄)-induced fibrosis(cirrhosis). In this study, it was investigated whether dried extract of 田螺(Concha Cipangopaludinae; CC) has liver functional improvement, antioxidative and antifibrotic effect in rats those were induced liver fibrosis by CCl₄ administration. The female Sprague-Dawley rats were divided into 3 groups(Normal, AC, AC-CC) and were observed in 6 weeks. Except for normal group, liver fibrosis(cirrhosis) in rats were developed by CCl₄ administration(0.8 ㎖/rat/week). And the rats were treated with prepared CC(p. o. 2 ㎖/day/rat). At the time of sacrifice, the liver, kidney and spleen were weighed and the ratio of organ weight/body weight was calculated. The MDA, hyp and biochemical parameters(AST, ALT, ALP, t-bili) were measured in sera and liver tissue of rats. The strong yellow color of urine was observed in all CCl₄-treated group compared with normal group, but jaundice didn't appear in CCl₄-treated group. The mortality of CCl₄-treated group is very low(<13%) during 6 weeks of observation time. The ratio of liver/body as well as the weight of liver in CCl₄-treated rats significantly increased compared with that in normal group(p<0.001). The level of clinical parameters in sera of all liver fibrosis(cirrhosis) developed rats were significantly higher than that of normal group(p<0.001-0.05). Especially the value of BUN, ALP, t-bilirubin in AC-CC group showed 20.9%, 19.6%, 47.9% lower than that in AC group. The content of hyp in CCl₄-treated rats was significantly higher than normal group(p<0.001～<0.05), and showed 12.2% lower value in the AC-CC group than AC group(p<0.05). The production of lipid peroxidation(MDA) in sera and liver tissue significantly increased under the fibrotic(cirrhotic) condition(p<0.001～<0.05). Especially the MDA value of AC-CC group in sera significantly 46.5% decreased compared with that of AC group(p<0.05), and the MDA value of AC-CC in liver tissue showed 21.4% lower than that of AC group. Concha Cipangopaludinae can be improved hepatic function, and maybe have effect of liver protection, antioxidation and antifibrosis.
Keywords
Concha Cipangopaludinae(田螺); liver fibrosis(cirrhosis); CCl₄; Malondi-aldehyde(MDA); hydroxyproline(hyp);
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  • Reference
1 /
[ Gerok W.(ed.) ] / Hepatologie :Urban & Schwarzenberg
2 /
[ Cotran-Humar R.(ed.) ] / Robbins pathologic basis of disease.(4th ed.)
3 茵蔯五&#33491;散의 白鼠 肝硬變에 對한 保護 및 回復作用. /
[ 金宇煥 ] / 博碩士學位論文
4 육울탕 및 산울탕이 膽道結紮과 Dimethylnitrosamine으로 誘發된 白鼠의 肝硬變症에 미치는 影響. /
[ 洪碩義 ] / 碩士學位論文
5 淸肺瀉肝湯의 治療效果에 關한 實驗的 硏究. /
[ 金定濟(外) ] / 慶熙韓醫大論文集
6 加減茵蔯蒿湯이 水浸液이 膽管結紮로 誘導된 肝損傷에 미치는 影響 /
[ 李建穆 ] / 博士學位論文
7 Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. /
[ Ohkawa H.;Ohishi N.;Yagi K. ] / Anal.Biochem.   DOI
8 On the role of lipid peroxidation in the pathogenesis of liver damage induced by long-standing cholestasis. /
[ Paola M.;Leonardizzi G.;Robino G.;Albano E.;Poli G.;Dianzani M. U. ] / Free Radic. Biol. Med.   DOI
9 Stimulation of collagen <TEX>${\alpha}1(Ⅰ)$</TEX> gene expression is associated with lipid peroxidation in hepatocellular injury: A link to tissue fibrosis? /
[ Bedossa P.;Houglum K.;Trautwein C.;Holstege A.;Chojkier M. ] / Hepatology
10 실험적 간경화(섬유화) 동물모델의 비교에 관한 연구 /
[ 김기영;김종수;유병수 ] / Kor. J. Lab. Anim. Sci.
11 梔子栢皮湯이 膽道結紮로 誘發된 白鼠의 肝硬變症에 미치는 影響. /
[ 李相祿 ] / 碩士學位論文
12 田螺 抽出物의 肝機能 改善과 抗纖維化 作用에 대한 硏究. /
[ 徐毅錫 ] / 博士學位論文
13 桂枝茯&#33491;丸이 四&#22633;化炭素로 因한 白鼠肝損傷에 미치는 影響. /
[ 申鎭&#29765; ] / 碩士學位論文
14 加減 小柴胡湯의 解熱 鎭痛 및 損傷肝에 미치는 影響. /
[ 趙鍾寬 ] / 碩士學位論文
15 Bleomycin-induced pulmonary fibrosis in rats: Biochemical demonstration of increased rate of collagen synthesis. /
[ Phan SH;Thrall RS;Ward PA. ] / Am. Rev Respir. Dis.   DOI
16 加味柴陣湯이 白鼠 肝損傷에 대한 效果 /
[ 王泰錫 ] / 博士學位論文
17 The hepatotoxic action of carbon tetrachloride stimulatory effect of carbon tetrachloride on lipid peroxidation in microsomal suspensions. /
[ Slater TF;Sawyer BC. ] / FEBS Lett.
18 Vitamin E dietary supplementation protects against carbon tetrachloride-induced chronic liver damage and cirrhosis. /
[ Parola M;Leonarduzzi G;Biasi F;Albano E;Biocca ME;Poli G;Dianzani MU. ] / Hepatology   DOI
19 Beyond cholesterol: Modifications of low-density lipoprotein that increase its atherogenicity. /
[ Steinberg D;Parthasarathy S;Carew TF;Khoo JC;Witztum JL. ] / N. Engl. J. Med.   DOI
20 Pathogenesis and treatment of photocutaneous manifestations of the porphyrias. /
[ Poh-Fitzpatrirk MB. ] / Semin. Liv Dis.   DOI
21 Chemistry and biochemistry of 4-hydroxynonenal, malomdialdehyde and related aldehydes. /
[ Esterbauer H;Schaur RJ;Zollner H. ] / free Rad. Biol. Med.   DOI
22 The flavonoid guercetin ameliorates liver damage in rats with biliary obstruction. /
[ Peres W;Tunon MJ;Collado PS, Herrmann S; Marroni N;Gonzalez-Gallego J. ] / J. Hepatol.
23 Carotenoids; Functions and fallacies. /
[ Thumham Dl. ] / Proc.Nutr Soc.   DOI
24 A pilot study of the effects of D-<TEX>${\alpha}$</TEX>-tocopherol on hepatic stellate cell activation in chronic hepatitis C. /
[ Houglum K;Venkataramani A;Lyche K;Chojkier M. ] / Gastroenterology.   DOI
25 Risk or angina Pectoris and Piasma concentrations of vitamin A, C and E and carotene. /
[ Riemersma RA;Wood DA;Macintyre CA;Elton RA;Gey KF;Oliver MF. ] / Lancet   DOI
26 Effect of antioxidants, reveratrol, quercetin, and N-acetylcysteine, on the funrtions of cultured rat hepatic stellate cells and kuffer cells /
[ Kawada N;Seki S;Inoue M;Kuroki T. ] / Hepatology.   DOI
27 Review of plants used in the treatment of liver disease : Part 1 /
[ Luper SA. ] / Altern. Med. Rev
28 人蔘이 CCI<TEX>$_4$</TEX> 中毒 흰쥐의 肝損傷에 미치는 影響에 관한 硏究 /
[ 崔榮植 ] / 碩士學位論文
29 人蔘이 CCI<TEX>$_4$</TEX> 中毒 흰쥐의 肝損傷에 미치는 影響에 관한 硏究. /
[ 崔榮植 ] / 碩士學位論文
30 葛根, 大棗 및 五味子의 肝機能 保護 效果에 관한 硏究. /
[ 李潤璟 ] / 博士學位論文
31 茵蔯蒿湯이 carbon tetrachlorid中毒으로 인한 白鼠肝臟損傷의 效果에 關한 實驗的 硏究. /
[ 裵文弘 ] / 慶熙大學校 論文集
32 木香調氣散과 解鬱調胃湯의 實驗的 肝硬變에 對한 效果 /
[ 朴庸權 ] / 碩士學位論文
33 當歸藥鍼 및 黃&#33450;藥鍼黃이 CCl<TEX>$_4$</TEX>에 의한 肝損傷의 恢復效果에 관한 實驗的 硏究. /
[ 金英吉 ] / 碩士學位論文
34 Antioxidant properties of colchicinr in acute carbon tetrachloride induced rat liver injury and its role in the resolution of established cirrhosis. /
[ Das D;Pemberton PW;Burrows PC;Gordon C; Smith A;McMahon Raymond FT;Warnes TW. ] / Biorhem. Biophysica. Acta.   DOI
35 香砂平胃散 및 當歸活血湯이 膽道結紮과Dimetylnitrosamine으로 誘發된 白鼠의 肝硬變症에 미치는 影響. /
[ 박대영 ] / 碩士學位論文
36 Effects of Sho-saiko-to, a Japanese herbal medicine, on hepatic fibrosis in rats. /
[ Shimizu I.;Ma Y. R. : Mizobuchi Y.;Liu F.;Miura T.;Nakai Y.;Yasuda M.;Shiba M.;Horie T.;Amagaya S.;Kawada N.;Hori H.;Ito S. ] / Hepatology   DOI
37 Detection of carbonyl functions in phospholipids of microsomes in CCl<TEX>$_4-$</TEX> and Br CCl<TEX>$_3-$</TEX>poisoned rats. /
[ Benedetti A;Fulceri R;Ferrali M;Ciccoli L;Esterbauer H;Comporti M. ] / Biochem. Biophys. Acta.   DOI
38 急性 肝損傷에 대한 丹蔘의 保護作用 /
[ 朴二圭 ] / 碩士學位論文
39 柴胡四物湯이CCl<TEX>$_4$</TEX>로 誘發된 白鼠의 肝損傷에 미치는 影響. /
[ 李在馥 ] / 碩士學位論文
40 黃&#33491;煎湯液이 肝損傷에 대한 防禦및 恢復에 미치는 影響. /
[ 姜秉淇 ] / 博士學位論文
41 Hepatic lipid peroxidation in vivo in rats with chronic iron overload. /
[ Bacon BR;Tavill AT;Brittenham GM;Park CH; Recknagel RO. ] / J. Clin. Invest.   DOI
42 A single method to determine nanogram levels of 4-hydroxyproline in biological tissue. /
[ Jamall I. S.;Finelli V. N. ] / Anal. Biochem.   DOI