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Cell Cycle Arrest by Sabaek-san is Associated with induction of Cdk Inhibitor p21 in Human Lung Cancer A549 Cells  

Kang Byong Ryeung (Department of Internal Medicine, Dongeui University and Research Institute of Oriental Medicine)
Oh Chang Sun (Department of Internal Medicine, Dongeui University and Research Institute of Oriental Medicine)
Lee Jae Hun (Department of Biochemistry, College of Oriental Medicine, Dongeui University and Research Institute of Oriental Medicine)
Choi Yung Hyun (Department of Biochemistry, College of Oriental Medicine, Dongeui University and Research Institute of Oriental Medicine)
Park Dong Il (Department of Internal Medicine, Dongeui University and Research Institute of Oriental Medicine)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.16, no.6, 2002 , pp. 1177-1183 More about this Journal
Abstract
We investigated the effects of Sabaek-san (SBS) water extract on the cell proliferation of human lung carcinoma A549 cells. SBS treatment resulted in the inhibition of cell proliferation in a concentration-dependent manner. This anti-proliferative effect of A549 cells by SBS treatment was associated with morphological changes such as membrane shrinking and cell rounding up. DNA flow cytometric histograms showed that population of G1 phase of the cell cycle was increased by SBS treatment in a concentration-dependent manner. SBS treatment induced a marked accumulation of tumor suppressor p53 and a concomitant induction of cyclin-dependent kinase (Cdk) inhibitor p21WAF1/CIP, which appears to be transcriptionally upregulated and is p53 dependent. In addition, SBS treatment resulted in down-regulation of cyclooxygenase-2 (COX-2) as determined by RT-PCR analysis. The present results indicated that SBS-induced inhibition of lung cancer cell proliferation is associated with the blockage of G1/S progression the induction of apoptosis.
Keywords
Sabaek-san(瀉白散); cell cycle; p53; p21; human lung cancer;
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