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Effect of Radix Polygoni Multiflori on Cultured Vascular Endotherial Cells Damaged by Xanthine Oxidase and Hypoxanthine  

Lee Yang Suk (Department of Medicine, Wonkwang University)
Kim Hyeong Su (Department of Medicine, Wonkwang University)
Son Young Woo (Department of Medicine, Wonkwang University)
Yoo Kyo Sang (Department of Medicine, Wonkwang University)
Lee Jung Hun (Department of Medicine, Wonkwang University)
Lee Kang Chang (Graduate School of Oriental Medicine, Wonkwang University)
Choi Kyu Chul (Wonkwang Heath Science College)
Shin Hong Chul (Department of Medicine, Wonkwang University)
Park Seung Taeck (Department of Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.16, no.4, 2002 , pp. 720-723 More about this Journal
Abstract
To clarify the vasculotoxicity of reactive oxygen intermediate(ROI) in cultured vascular endotherial cells(VEC), of mouse, cytototoxicity was measured by MTS assay after VEC was incubated to 10～80mU/ml xanthine oxidase(XO) and hypoxanthine(HX) for 2 hours. and also, the protective effect of Radix Polygoni Multiflori(RPM) was determined by MTT assay in these cultrures. Cell viability was positively decreased dose-, and time-dependently, after the treatment with 40mU/ml XO/0.1 mM HX to cultured VEC for 2 hours. In the vasculoprotective effect of RPM on the toxicity induced by XO/HX, RPM prevented the XO/HX-induced cytotoxicity in these cultures. From above the results, it suggests that XO/HX is toxic in cultured VEC and herb extract, RPM has protective effect against the vasculotoxicity induced by XO/HX.
Keywords
xanthine oxidase; vascular endotherial cells; Radix Polygoni Multiflori;
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