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Protective Effect of Puerariae radix Against Ethanol-induced Apoptosis on Human Neuroblastoma Cell Line SK-N-MC  

Koo Gyo Sung (Oriental Medicine Research Institute, Kyungwon University)
Cho Son Hae (Oriental Medicine Research Institute, Kyungwon University)
Jang Mi Hyean (Department of Physiology. College of Medicine, Kyunghee University)
Kim Chang Ju (Department of Physiology. College of Medicine, Kyunghee University)
Kim Ee Hwa (Department of Meridinology, College of Oriental. Medicine, Semyung University)
Lee Choong Yeol (Oriental Medicine Research Institute, Kyungwon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.16, no.3, 2002 , pp. 602-608 More about this Journal
Abstract
To investigate whether Puerariae radix (PR) possesses protective effect against ethanol (EtOH)-inducecl apoptosis in the central nervous system, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, flow cytometric analysis, DNA fragmentation assay. and reverse transcription-polymerase chain reaction (RT-PCR) were performed on human neuroblastoma cell line SK-N-MC. Morphological and biochemical analyses demonstrated that SK-N-MC cells treated with EtOH exhibit classical apoptotic features. On the other hand, cells pre-treated with PR prior to EtOH exposure showed decreased occurrence of classical apoptotic features. In addition, it was shown that PR pre-treatment inhibits EtOH-induced increases in the levels of mRNA expression of bax and caspase-3, while it further enhances the level of bcl-2 expression. These results suggest that PR may exert protective effects against EtOH-induced apoptosis in human neuroblastoma cells.
Keywords
Puerariae radix; ethanol; apoptosis; neuroblastoma;
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