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Effects of Jingansikpung-tang Water Extract on LDH Activity of Cultured Spinal Sensory Neurons Damaged by GO  

Park Kwang Su (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Kwon Kang Beam (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Seong Eun Kyung (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Song Yong Sun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University)
Ryu Do Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.16, no.3, 2002 , pp. 563-566 More about this Journal
Abstract
To evaluate the effect of Jingansikpung-tang(JST) water extract on cultured mouse spinal sensory neuron which was inhibited by glucose oxidase(GO)-induced cytotoxicity, MTT and LDH activity assay were carried out after the cultured mouse spinal sensory neuron were pre-incubated with various concentrations of JST extract for 3 hours prior to exposure of GO. The results obtained were as follows: GO, a oxygen radical, decreased the survival rate of the cultured mouse spinal sensory neuron cells on MTT assay. JST water extract have efficacy of decreasing LDH activity increasing by GO in cultured mouse spinal sensory neuron. From above the results, it is concluded that JST water extract has marked efficacy as a treatment for the damages caused in the GO-mediated oxidative process.
Keywords
Jingansikpung-tang(鎭肝熄風湯); glucose oxidase(GO); MTT; LDH activity;
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