Browse > Article

Enhancement of Immune Response by New Herb Mixture, APA-01, in Mice  

Lee Young Sun (Efficacy and Safety Research Center for Traditional Oriental Medicines, Kyungsan University, Kyongbuk Technopark)
Han Ok Kyung (Efficacy and Safety Research Center for Traditional Oriental Medicines, Kyungsan University, Kyongbuk Technopark)
Park Chan Woo (Kyongju Oriental Hospital, Gyeongju)
Jeon Tae Won (Efficacy and Safety Research Center for Traditional Oriental Medicines, Kyungsan University, Kyongbuk Technopark)
Lee Eun Sil (Efficacy and Safety Research Center for Traditional Oriental Medicines, Kyungsan University, Kyongbuk Technopark)
Shin Sang Woo (College of Oriental Medicine, Kyungsan, University)
Kim Kwang Joong (College of Oriental Medicine, Kyungsan, University)
Kim Hyo Jung (Efficacy and Safety Research Center for Traditional Oriental Medicines, Kyungsan University, Kyongbuk Technopark, College of Oriental Medicine, Kyungsan, University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.16, no.3, 2002 , pp. 483-489 More about this Journal
Abstract
APA-01, which is an aqueous extract of five Chinese herbs, is a modified formula of Huoxiang-Zhengqi-San. The effect of new herb extract on immune response was investigated. The parameter examined to assess apparent immune response of APA-01 in mice included changes of body weight, relative weight of immune organs, cell proliferation and cytokine gene expression. The body weight and relative weight of immune organs were not significantly changed among the tested groups. In the spleen cell prolijeration assay, APA-01 increased the cell proliferation in a dose-dependent manner. Methotrexate (MTX), an agent of immune suppression, inhibited the spleen cell proliferation (IC/sub 50/: 800㎍/㎖). However, APA-01 significantly inhibited the suppression of mouse spleen cell proliferation. Therefore, it seems that APA-01 has a reducing effect of immune suppression. Immunomodulatory effect of APA-01 was further investigated using reverse transcription polymerase chain reaction (RT-PCR) in mouse spleen cells. In RT-PCR test, APA-01 enhanced the expression of cyclooxygenase-2 (COX-2) mRNA in a dose-dependent manner. In spite of immune suppression by MTX, COX-2 mRNA was induced by co-treatment with APA-01. These results suggest that APA-01 stimulates the proliferation of spleen cells, regulates the expression of COX-2 mRNA, and accelerates the recovery of inhibition of spleen cell proliferation induced by MTX, thus providing the immunological basis for clinical benefit of APA-01.
Keywords
APA-01; methotrexate; cytokine; RT-PCR; cell proliferation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 당귀 추출물이 면역계에 미치는 영향(II) /
[ 조성기;윤연숙;박승용;문은이;박은규 ] / 대한면역학회지
2 황기도홍탕(黃耆桃紅湯)의 항종양 효과 및 면역조절반응에 관한 연구 /
[ 전병훈;이진홍;정우열 ] / 동의병리학회지
3 황기(黃耆)의 간기능 보호 성분 /
[ 백남인;김영숙;경종수;박기현 ] / 생약학회지
4 당귀 추출물이 면역계에 미치는 영향 /
[ 조성기;문혜선;윤연숙;홍석일;함용호;정인성;박은규 ] / 대한면역학회지
5 어성초 및 상국음이 면역기능에 미치는 영향 /
[ 길영성;정승기;이형구 ] / 대한한의학회지
6 IL-6 mRNA Expression in Mouse Peritoneal Macrophages and NIH3T3 Fibroblasts in Response to Candida albicans /
[ Lee YS;Kim HS;Kim SK;Kim SD ] / J Microbiol Biotechnol.
7 Effect of shi-ka-ron and Chinese herbs on cytokine production of macrophage in immunocom-promised mice /
[ Jin R;Wan LL;Mitsuishi T;Sato S;Akuzawa Y;Kodama K;Kurashige S ] / Am J Chin Med.   ScienceOn
8 A traditional Chinese herbal medicine, ren-shen-yang-rong-tang (Japanese name: ninjin-yoei-to) augments the production of granulocyte-macrophage colony-stimulating factor from human peripheral blood mononuclear cells in vitro /
[ Okamura S;Shimoda K;Yu LX;Omori F;Niho Y ] / Int J Immunopharmacol.   ScienceOn
9 신수혈의 침자극과 황기약침이 실험용 생쥐의 면역활성물질인 cytokine의 IL-6 발현에 미치는 영향 /
[ 김종수;신상습;김철호;박선동;박원환 ] / 대한침구학회지
10 황기가 면역세포의 기능에 미치는 영향 /
[ 송봉근;이언정;김형균;진선두;김성재;김동혁 ] / 대한본초학회지(본초분과학회지)
11 식품을 이용한 화학적 암예방 /
[ 서영준 ] / 식품과학과 산업
12 인삼 엑기스의 경구 면역 관용에 관한 연구 /
[ 배만종 ] / 한국식품영양학회지
13 Effects of astragali radix extract on carcinogenesis, cytokine production, and cytotoxicity in mice treated with a carcinogen, N-butyl- N'-butanolnitrosoamine /
[ Kurashige S;Akuzawa Y;Endo F ] / Cancer Invest.
14 13종 허브추출물의 수퍼옥사이드 소거능과 세포독성 및 면역증강 효과의 in vitro계 검색 /
[ 정하열;김현배 ] / 한국식품과학회지
15 공진난 약침자극이 혈액 및 각 면역 조직의 임파구와 CD4+ T세포에 미치는 영향 /
[ 김용석;이재동;김창환;최도영;박동석;남상수;강성길;이윤호;안병철;최용태;고형균;주정주 ] / 대한침구학회지
16 Enhancement of the immune response in mice by Astragalus membranaceus extracts /
[ Zhao KS;Mancini C;Doria G ] / Immunopharmacology   ScienceOn
17 신수혈의 침자극과 황기약침이 실험용 생쥐의 면역활성물질인 IL-1 <TEX>$\alpha$<\TEX>의 유전자발현에 미치는 영향 /
[ 홍문엽;박원환;신상습;최달영;이태균 ] / 동의병리학회지
18 항암 면역 증강 작용이 있는 인삼 다당체에 의한 cytokine mRNA의 발현 /
[ 김기환;이인란;정인성;정희용;윤연숙 ] / 고려인삼학회지
19 /
[ 安德均 ] / 면역과 한방
20 Chinese medicinal herbs reverse macrophage suppression induced by urological tumors /
[ Rittenhouse JR;Lui PD;Lau BH ] / J Urol.
21 Anticarcinogenic effects of shikaron, a preparation of eight Chinese herbs in mice treated with a carcinogen, N-butyl-N'-butanolnitrosoamine /
[ Kurashige S;Jin R;Akuzawa Y;Endo F ] / Cancer Invest.   ScienceOn
22 Effects of Chinese herbs on macrophage functions in N-butyl-N-butanolnitrosoamine treated mice /
[ Jin R;Kurashige S ] / mmunopharmacol Immunotoxicol.   ScienceOn
23 생약제가 면역세포 활성화에 미치는 영향 /
[ 이인선;하영득 ] / 한국식품영양과학회지
24 상황버섯 추출물이 정상 마우스와 cyclophosphamide로 처리된 마우스의 체액성 면역기능에 미치는 영향 /
[ 표명윤;양기숙;현수미 ] / 응용약물학회지
25 Differences in immunomodulating effects between wild and cultured Panax ginseng /
[ Mizuno M;Yamada J;Terai H;Kozukue N;Lee YS;Tsuchida H ] / Biochem Biophys Res Commun.   ScienceOn
26 /
[ 최윤정 ] / 사군자탕 및 사물탕이 methotrexate로 유발된 흰쥐의 면역기능저하에 미치는 영향
27 황기수침(黃耆水鍼)이 Methotrexate 를 투여한 생쥐의 면역반응에 미치는 영향 /
[ 김창환;고형균;배원영 ] / 대한침구학회지
28 하수오가 methotrexate로 유도된 흰쥐의 면역기능저하에 미치는 영향 /
[ 김일영;이상재;김광호 ] / 대한예방한의학회지
29 인삼 사포닌이 인간면역계 사이토카인 유전자의 발현에 미치는 영향 /
[ 박종욱;한인숙;서성일;백원기;서민호;배지현;최병길 ] / 고려인삼학회지
30 마이토젠 및 림포카인이 마우스 비장 세포 임파구 및 사람 말초 혈액 임파구 증식 반응에 미치는 영향 /
[ 정태준;김정목;조양자;정용훈;임대철 ] / 대한면역학회지
31 Involvement of Protein kinase C-r in IL-1b induced cyclooxygenase-2 expression in human pulmonary epithelial cells /
[ Lin CH;Sheu SY;Lee HM;Lee WS;Ko WC;Sheu JR ] / Mol. Pharmaco.
32 Cyclooxygenase-1 and cyclooxygenase-2 expression in rat kidney and adrenal gland after stimulation with systemic lipopolysaccharide: in situ hybridization and immunocytochemical studies /
[ Ichitani Y;Holmberg K;Maunsbach AB;Haeggstrom JZ;Samuelsson B;De Witt D; Hokfelt T ] / Cell Tissue Res.   ScienceOn