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Effect of 1,2,3,4,6-penta-O-galloyl-$\beta$-D-glucose isolated from Galla Rhois on gap junctional intercellular communication and antiangiogenic activity  

Huh Jeong Eun (Department of Oncology, Graduate School of East-West Medial Science, KyungHee University)
Lee Hyo Jung (Department of Oncology, Graduate School of East-West Medial Science, KyungHee University)
Song Gyu Yong (Department of Oncology, Graduate School of East-West Medial Science, KyungHee University)
Cha Bae Cheon (Division of Applied Animal Science, Sangjj University)
Kim Han Sung (College of Oriental Medicine, Daejon University)
Yoo Dong Youl (College of Oriental Medicine, Daejon University)
Ryu Shi Yong (Korea Research Institute of Chemical Technology)
Kim Sung Hoon (Department of Oncology, Graduate School of East-West Medial Science, KyungHee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.16, no.3, 2002 , pp. 452-457 More about this Journal
Abstract
Galla Rhois is a gallnut of Rhus javanica Linne used for treatment of diarrhea, hemorrhage, cough, leukorrhea and toxic tumor etc in oriental medicine. For the evaluation of antitumor effect of Galla Rhois, activity based fractionation was done. We isolated an effective compound and identified 1,2,3,4,6-penta-O-galloyl-β-D-glucose(PGG) by photometric analysis such as NMR and MASS. Then, we studied the angiogenic activity of PGG. It showed a cytotoxicity against SK-OV-3, SK-OV-3, HT1080 with IC/sub 50/ of 50 ug/ml approximately. It also effectively inhibited proliferation of HUVEC cells treated by bFGF to 30% of control at 20 ug/ml and cell migration to 80% at 10 ug in a dose dependent fashion. Tube formation of HUVEC cells on matrigel was effectively suppressed from 2.5 ug/ml of concentration by PGG. Moreover, it effectively recovered the dysfunction of gap junctional intercellular communication in WB-F344 rat liver epithelial cells caused by hydrogen peroxide at 4 ug/ml suggesting it potently can inhibit tumor promotion. Taken together, it indicates 1,2,3,4,6-penta-O-galloyl- β -D-glucose has antiangiogenic activity.
Keywords
Galla Rhois; 1,2,3,4,6-penta-O-galloyl-β-D-glucose; migration; invasion; gap junction; tube formation;
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