[KSCI] Korea Science Citation Index Service

Mechanism of Relaxation of Rat Aorta by Scopoletin; an Active Constituent of Artemisia Capillaris

Kwon Eui Kwang (Professional Graduate School of Oriental Medicine, Wonkwang University)
Jin Sang Sik (Professional Graduate School of Oriental Medicine, Wonkwang University)
oChoi Min H (Professional Graduate School of Oriental Medicine, Wonkwang University)
Hwang Kyung Taek (Professional Graduate School of Oriental Medicine, Wonkwang University)
Shim Jin Chan (Professional Graduate School of Oriental Medicine, Wonkwang University)
Hwang Il Taek (Professional Graduate School of Oriental Medicine, Wonkwang University)
Han Jong Hyun (Professional Graduate School of Oriental Medicine, Wonkwang University)

Publication Information

Journal of Physiology & Pathology in Korean Medicine / v.16, no.2, 2002 , pp. 389-396 More about this Journal

Abstract

In the present work, we examined the mechanism of vasorelaxant effect of scopoletin, an active constituent of Artemisia capillaris on rat thoracic descending aortic rings. Scopoletin induced a concentration-dependent relaxation in rat thoracic descending aortic rings pre-contracted with phenylephrine (EC/sub 50/ = 238.94±37.4 μM), while it was less effective in rat thoracic descending aortic rings precontracted with high potassium solution (KCI 30 mM). Vasorelaxation by scopoletin was significantly inhibited after endothelial removal, but recovered at high concentration. Pretreatment of rat thoracic descending aortic rings with N/sup G/-nitro-L-arginine (100 μM), a nitric oxide synthase inhibitor, and atropine (1 μM), a muscarinic receptor antagonist, significantly inhibited scopoletin-induced relaxation of rat thoracic descending aortic rings. Neither indomethacin (3 μM), an inhibitor of cydooxygenase, nor propranolol (1 μM), a β -adrenoceptor antagonist, modified the effect of scopoletin. The combination of N/sup G/ -nitro-L-arginine (100 μ M) and miconazole (10 μ M), an inhibitor of cytochrome P 450, did not modify the effect of scopoletin, when compared with pretreatment with N/sup G/-nitro-L-arginine(100 μM) alone. Vasorelaxant effect of scopoletin was inverted by pretreatment with diltiazem (10 μM), a Ca/sup 2+/-channel blocker, at low concentration, while restored at high concentration. Apamin (K/sub ca/-channel blocker, 1 μM), 4-aminopyridine (4-AP, K/sub v/-channel blocker, 1 mM), and tetrodotoxin (TTX, Na/sup +/-channel blocker 1 μM) potentiated the vasorelaxant effect of scopoledn, but glibendamide (K/sub ATP/-channel blocker, 10 μM), tetraetylammonium(TEA, non-selective K-channel blocker, 10 mM) did not affect the relaxation of scopoletin. Free radical scavengers (TEMPO, catalase, mannitol) did not modify vascular tone. These results suggest that nitric oxide, Ca/sup 2+/ -channels play a role in endothelium-dependent relaxations to scopoletin in rat aortas, that apamin, 4-AP, TTX but not glibenclamide, TEA potentiated relaxation to scopoletin mediated by these channels, and that free radicals do not concern to the vasorelaxant effect of scopoletin.

Keywords

Artemisia capillaris; scopoletin; endothelium-derived relaxing factor(EDRF); nitric oxide(NO); endothelium-derived hyperpolarizing factor(EDHF); potassium channel; calcium channel; β-adrenoceptor;

Citations & Related Records

Reference

1	Flow activates an endothelial potassium channel to release an endogenous nitrovasodilator. / [ Coke, J. P.;Rossitch, E. Jr.;Andon, N. A.;Dazu, V. J. ] / J. Clin. Invest. DOI
2	Ca<TEX>$^{2+}$</TEX>-stimulated mitochondrial reactive oxygen species generation and permeability transition are inhibited by dibucaine or Mg<TEX>$^{2+}$</TEX>. / [ Kowaltowski, A. J.;Naia-da-silva, E. S.;Castilho, R. F.;Vercesi, A. E. ] / Arch. Biocem. Biophys. DOI
3	A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: Applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. / [ Zhou, M.;Diwu, Z.;Voloshina, N. V.;Haugland, R. P. ] / Anal. Biochem. DOI
4	NADPH oxidation catalyzed by the peroxidase/<TEX>$H_{2}O_{2}$</TEX> system: Guaiacol-mediated and scopoletin-mediated oxidation of NADPH to NADP+. / [ Michot, J. L.;Virion, A.;Deme, D.;De Prailaune, S.;Pommier, J. ] / Eur. J. Biochem. DOI
5	Primary endothelial dysfunction: Atherosclerosis. / [ Shimokawa, H. ] / J. Moll. cell. cardiol. DOI
6	Calcium-dependent and -independent activation of the endothelial NO synthase. / [ Fleming, I.;Bauersachs, J.;Busse, R. ] / J. Vasc Res. DOI
7	NO: the primary EDRF. / [ Fleming, I.;Busse, R. ] / J. Moll cell cardiol. DOI
8	The alternative: EDHF / [ F l tou, M.;Vanhoutte, P. M. ] / J. Moll cell cardiol. DOI
9	Hydroxyl radical - a mediator of acetylcholine- induced vascular relaxtion. / [ Prasad, K.;Bharadwaj, L. A. ] / J. Moll cell cardiol. DOI
10	Time course of changes in endothelium -dependent and -independent relaxation of chronically diabetic aorta: role of reactive oxygen species. / [ Karasu, C. ] / Eur. J. Pharmacol. DOI
11	U. Nitric oxide in the pathogenesis of vascular disease. / [ Li, H. Forstermann ] / J. pathol. DOI
12	/ [ Shin, M.K. ] / Colourful Clinical Materia Medica
13	/ [ Chung, B.S.;Shin, M.K. ] / Native Herb (Crude Drug) Encyclopedia [a book of Plants]
14	Studies on choleretic constituent in Artemisia capillaris THUNB. / [ Okuno, I.;Uchida, K.;Nakamura, M.;Sakurawi, K. ] / Chem. Pharm. Bull. DOI
15	In eNOS knockout mice skeletal muscle arteriolar dilation to acetylchorine is mediated by EDHF. / [ Huang, A.;Sun, D.;Smith, C. J.;Counetta, J. A.;Shesely, E. G.;Koller, A.;Kaley, G. ] / Am. J. Physiol. Heart circ. Physiol.
16	Mechanism of the hypotensive effect of scopoletin isolated from the fruit of Tetrapleura tetraptera. / [ Ojewole, J. A. O.;Adesina, S. K. ] / Planta Med. DOI
17	Cardiovascular and neuromuscular action of scopoletin from the fruit of Tetrapleura tetraptera. / [ Ojewole, J. A. O.;Adesina, S. K. ] / Planta Med. DOI
18	Transient and steady-state kinetics of the oxidation of scopoletin by horseradish peroxidase compounds Ⅰ, Ⅱ and Ⅲ in the presence of NADH. / [ Marquez, L. A.;Dunford, H. B. ] / Eur. J. Biochem. DOI
19	Components of acetylcholine-induced dilation in isolated rat arterioles. / [ Bakker, E. N. T. P.;Sipkema, P. ] / Am. J. Physiol.
20	Endothelium-derived nitric oxide: action and properties. / [ Ignarro, L. J. ] / FASEB J.
21	/ [ Rang, H. P.;Dale, M. M.;Ritter, J. M. ] / Pharmacology.
22	Endothelium -dependent relaxation resistant to NG-nitro-L-arginine in rat aorta. / [ Hadake, K.;Wakabayashi, I.;Hishda, S. ] / Eur. J. Pharm. DOI
23	A major role for prostacyclin in nitric oxide-induced occular vasorelaxation in the piglet. / [ Hardy, P.;Abran, D.;Hou, X.;Lahaie, I.;Peri, K. G.;Asselin, P.;Varma, D. R.;Chemtob, S. ] / Cicr Res DOI
24	Endothelium-derived hyperpolarizing factor - fact or fiction? / [ Hecker, M. ] / News in the Physiological science.
25	Scopoletin: an inducible nitric oxide synthesis inhibitory active constituent from Artemisia feddei. / [ Kang, T. H.;Pae, H. O.;Jeong, S. J.;Yoo, J. C.;Choi, B. M.;Jun, C. D.;Chung, H. T.; Miyamotm, R.;Higuchi, R.;Kim, Y. C. ] / Planta Med.
26	In vitro inducible nitric oxide synthesis inhibitory active constituents from Fraxinus rhynchophylla. / [ Kang, T. H.;Pae, H. O.;Ko, Y. S.;Yoo, J. C.;Choi, B. M.;Jun, C. D.;Chung, H. T.; Inagaki, M.;Higuchi, R.;Kim, Y. C. ] / Planta Med. DOI
27	Choleretic effect of Artemisia capllaris extract in rats. / [ Okuno, I.;uchida, K.;Kadowaki, M.;Akahori, A. ] / Japan. J. Pharmacol. DOI
28	Production of holeretic substances in the capitulum, leaf and stem of Artemisia capillaris during the plant growth cycle. / [ Ikenaga, T.;Hizaco, M.;Tajima, M.;Nakayama, K. ] / Biol. Pharmacol. Bull. DOI
29	Hepatoprotective activity of scopoletin, a constituent of Solarum lyratum. / [ Kang, S. Y.;Sung, S.H.;Park, J. H.;Kim, Y. C. ] / Arch. Pharmacol. Res. DOI
30	Antihepatotoxic principles of Artemisia capillaris buds. / [ Kiso, Y.;Ogasawara, S.;Hirota, K.;Watanabe, N.;Oshima, Y.;Konno, C.;Hikino, H. ] / Planta Med.
31	Agonist -dependent difference in the relationship between cytosolic Ca<TEX>$^{2+}$</TEX> level and release of vascular relaxing factor in the endothelium of rabbit aortic valve. / [ Amano, K.;Hori, M.;Ozaki, H.;Karaki, H. ] / Eur. J. Pharmacol. DOI
32	Hydrogen peroxide release from human eosinophils on fibronectin: scopoletin enhances eosinophil activation. / [ Raible, D. G.;Mohanty, J. G.;Jaffe, J. S.;Stella, H. J.;Sprenkle, B. E.;Glaum, M. C.; Schulmann, E. S. ] / Free radical biology & Medicine. DOI
33	Etomidate and thiopental inhibit the release of endothelium-derived hyperpolarizing factor in the human renal artery. / [ Kessler, P.;Lischke, V.;Hecker, M. ] / Anesthesiology. DOI
34	Calcium-dependent nitric oxide synthesis in endothelial cytosol is mediated by calmodulin. / [ Busse, R.;Műlsch, A. ] / FEBS lett. DOI