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Effect of Rhizoma Gastrodiae on Cultured Spinal Motor Neurons Damaged by Oxygen Radicals  

Son IL Hong (School of Medicine, Wonkwang University)
Lee Jung Hun (School of Medicine, Wonkwang University)
kim Sang Su (Institution of Wonkwang Science, Wonkwang University)
Lee Kang Chang (Department of Graduate School of Oriental Medicine, Wonkwang University)
Lee Young Mi (School of Medicine, Wonkwang University)
Hong Gi Youn (School of Medicine, Wonkwang University)
Moon Hyung Bae (School of Medicine, Wonkwang University)
Seo Eun A (Medicinal Resources Research Center, Wonkwang University)
Han Du Seok (College of Dental Medicine, Wonkwang University)
Shin Min Kyo (College of Oriental Medicine, Wonkwang University)
Song Ho Joan (College of Oriental Medicine, Wonkwang University)
Park Seung Taeck (Institution of Wonkwang Science, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.16, no.2, 2002 , pp. 262-266 More about this Journal
Abstract
In order to elucidate the mechanism of cytotoxic effect of oxygen radicals on cultured mouse spinal motor neurons, the neurotoxicity induced by hydrogen peroxide(H₂O₂) was evaluated by MTT assay. The neuroprotective effect of Rhizoma Gastrodiae(RG) against H₂O₂-mediated neurotoxicity was also examined in these cultures by SRB assay. The results were as follows : The value of lethal concentration 50(LC50) of H₂O₂ was estimated at a concentration of 30 uM in these cultures. Cell viability of cultured mouse spinal motor neurons was remarkably decreased by H₂O₂-induced neurotoxicity in a dose- and time-dependent manner. RG was remarkably effective in blocking the neurotoxicity induced by H₂O₂ at a concentration of 120 μM as determined by SRB assay. From above the results, it is suggested that H₂O₂ induce neurotoxicity, and the selective herbal extracted RG was very effective in blocking H₂O₂-mediated neurotoxicity on cultured mouse spinal motor neurons.
Keywords
Oxygen radicals; Cultured spinal motor neuron; Rhizoma Gastrodiae;
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