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Effects of Moxibustion to Zusanli(ST36) on Alteration of Natural Killer cell Activity in rat spleen  

Choi Gi Soon (Department of East-West Medicine, Graduate School, Kyunghee University)
Oh Sang Deog (Department of East-West Medicine, Graduate School, Kyunghee University)
Han Jae Bok (Department of East-West Medicine, Graduate School, Kyunghee University)
Lee Gi Seog (Department of East-West Medicine, Graduate School, Kyunghee University)
Park Joan Ha (Department of East-West Medicine, Graduate School, Kyunghee University)
Bae Hyun Su (Department of Physiology, College of Oriental Medicine, Kyunghee University)
Jung Sung Ki (Department of Internal Medicine, College of Oriental Medicine, Kyunghee University)
Ahn Hyun Jong (Department of Microbiology, College of Medicine, Kyunghee University)
Cho Young Wuk (Department of Physiology, College of Medicine, Kyunghee University)
Min Byung Il (Department of East-West Medicine, Graduate School, Kyunghee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.16, no.2, 2002 , pp. 233-238 More about this Journal
Abstract
Moxibustion is one of major healing technique in oriental medicine. It has been widely used in many diseases such as rheumatoid arthritis, Hashimoto disease, breech presentation, etc. However, till now, effects of moxibustion on NK cell activity and relations between sympathetic nerve system(SNS) and the immune alteration induced by moxibustion were not well studied. This study was designed to evaluate effects of moxibustion on NK cell activity and the intervention of SNS in the alteration of NK cell activity induced by moxibustion. Splenic NK cytotoxity was measured in a standard 4-h 51Cr release assay. We measured the NK cytotoxity at after moxibustion stimulation for 1,3,5, and 7 days, and also measured the NK cell cytotoxity after 3 and 7 days burn stimulation with similar temperature. IL-2, IL-4, INF-γ in serum were measured by rat IL-2, IL-4, INF-γ ELISA TEST KIT. To evaluate the effects of sympathectomy on alteration of NK cell cytotoxity, 6-hydroxydopamine(6-OHDA : 5Omg/kg) was used. We showed that NK cell activity of moxibustion stimulation group increased at the 3rd day, and declined at 7th day in comparison with that of contol group. In moxibustion stimulation group, NK cell activity of 3 day stimulation group was significantly higher than sham group. On the contrary, in burn stimulation group, NK cell activity was significantly higher than that of sham groups at 3rd, 7th days. Patterns between moxibustion and burns were different. INF- γ level of 3 days moxibustion stimulation group significantly higher than sham group. IL 2 level among groups were not different. IL-4 was not detected in serum with this method. Sympathectomy abolished the NK cell activity alteration induced by moxibustion. The results suggest that moxibustion induces the alteration of NK cell activity, along with INF-γ and SNS is related to these effects.
Keywords
Moxibustion; NK cell activity; INF-gamma; sympathectomy;
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