Browse > Article

Puerariae radix increases Alcohol-induced Suppressed Cell Proliferation and Expression of Nitric Oxide Synthase in Dentate Gyrus of Rats  

Jang Mi Hyean (Department of Physiology, College of Medicine, Kyunghee University)
Lee Taeck Hyun (Department of Physiology, College of Medicine, Kyunghee University)
Shin Min Chul (Department of Physiology, College of Medicine, Kyunghee University)
Lim Baek Vin (Department of Physiology, College of Medicine, Kyunghee University)
Kim Hyun Bae (Department of Physiology, College of Medicine, Kyunghee University)
Lim Sabina (East-West Medial Research Institute, Kyunghee University)
Kim Jin Woo (East-West Medial Research Institute, Kyunghee University)
Lee Cheong Yeal (Department of Physiology, College of Oriental Medicine, Kyungwon University)
Kim Ee Hwa (Department of Meridianology, College of Oriental Medicine, Semyung University)
Kim Chang Ju (East-West Medial Research Institute, Kyunghee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.16, no.1, 2002 , pp. 192-196 More about this Journal
Abstract
Traditionally, aqueous extracts of Puerariae radix had been used for the treatment of alcohol-related problems. In the present study, the effect of Puerariae radix on cell proliferation and expression of nitric oxide synthase (NOS) in the dentate gyrus of alcohol-intoxicated rats were investigated via 5-bromo-2' -deoxyuridine (BrdU) immunohistochemistry and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry, respectively. Sprague-Dawley rats weighing 150 ± 10 g were divided into four groups: the control group, the Puerarias radix-treated group, the alcohol-treated group, and the alcohol- and Puerariae radix-treated group. The numbers of both BrdU-positive and NADPH-d-positive cells in the dentate gyrus were inhibited significantly by alcohol administration, while Puerariae radix treatment was shown to increase those numbers. In this study, it was revealed that Puerariae radix possesses protective effect against alcohol-induced suppressed new cell formation and NOS expression in the dentate gyrus. Based on the results, it is possible that NO, which might play an important role in the regulation of cell proliferation, is a major target of the toxic effects of alcohol.
Keywords
Puerariae radix; alcohol; dentate gyrus; 5-bromo-2' -deoxyuridine; nicotinamide adenine dinudeotide phosphate-diaphorase;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Glial-derived neurotrophic factor (GDNF) prevents ethanol-induced apoptosis and JUN kinase phosphorylation. /
[ McAlhany Jr, R.E.;West, J.R.;Miranda, R.C. ] / Dev Brain Res   DOI
2 Ethanol-induced apoptotic neurodegeneration and fetal alcohol syndrome. /
[ Ikonomidou, C.;Bittigau, P.;Ishimaru, M.J.;Wozniak, D.F.;Koch, C.;Genz, K.;Price, M.T.;Strfovska, V.;Horster, F.;Tenkova, T.;Dikranian, K.;Olney, J.W. ] / Science   DOI   ScienceOn
3 Memory deficits subsequent to chronic consumption of alcohol in mice: an analysis based on spontaneous alteration behavior. /
[ Beracochea, D.;Jaffard, R. ] / Behav Brain Res   DOI
4 Nitric oxide signaling in the central nervous system. /
[ Garthwaite, J.;Boulton, C.L. ] / Annu Rev Physiol   DOI
5 Ethanol can inhibit nitric oxide production. /
[ Persson, M.G.;Gustafsson, L.E. ] / Eur J Pharmacol   DOI
6 Morphological bases for a role of nitric oxide in adult neurogenesis. /
[ Moreno-López, B.;Noval, J.A.;González-Bonet, L.G.;Estrada, C. ] / Brain Res   DOI
7 Developmental expression of nitric oxide synthase isoformⅠand Ⅲ in chick retina. /
[ Gorueau, O.;Regnier-Ricard, F.;Jonet, L.;Jeanny, J.C.; Courtois, Y.;Chany-Fournier, F. ] / J Neurosci Res   DOI
8 Nitric oxide: a physiologic messenger molecule. /
[ Bredt, D.S.;Snyder, S.H. ] / Annu Rev Biochem   DOI
9 In vivo neurogenesis in the adult brain : regulation and functional implications. /
[ Fuchs, E.;Gould, E. ] / Eur J Neurosci   DOI
10 Ethanol-induced cell death in cultured rat astroglia. /
[ Holownia, A.;Ledig, M.;Mënez, J.F. ] / Neurotoxicol Teratol   DOI
11 Differential changes in neuropeptide Y and nicotinamide adenine dinucleotide phosphate-diaphorase- positive neurons in rat hippocampus after kainic acid- induced seizure. /
[ Park, C.;Kang, M.;Kang, K.;Lee, J.;Kim, J.;Yoo, J.;Ahn, H.;Huh, Y. ] / Neurosci Lett   DOI
12 Alcohol-induced inhibition on the expression of nitric oxide synthase in the cerebral cortex of Sprague-Dawley rats. /
[ Jang, M.-H.;Kim, C.-J.;Chung, J.-H.;Kim, Y.;Kim, E.-H.;Kang, B.-N. ] / Kor J Stress Res
13 The effects of Artemisia capillaries herbal acupuncture on ethanol-induced apoptosis in neuroblastoma cell line. /
[ Kim, E.-H.;Kim, Y.-H.;Jang, M.-H.;Chung, J.-H.;Kim, C.-J. ] / J Kor Orien Med
14 Preventive mechanisms of Artemisia capillaries extract on ethanol-induced apoptosis in neuroblastoma cell line. /
[ Kim, E.-H.;Lee, E.-Y.;Jang, M.-H.;Kim, Y.-J.;Kim, C.-J.;Chung, J.-H.;Seo, J.-C.;Kim, Y. ] / J Kor Acupunc & Moxibus Soci
15 Protective effects of Puerariae flos against ethanol-induced apoptosis on human neuroblastoma cell line SK-N-MC. /
[ Jang, M.-H.;Shin, M.-C.;Kim, Y.-J.;Chung, J.-H.;Yim, S.-V.;Kim, E.-H.;Kim, Y.;Kim, C.-J. ] / Jpn J Pharmacol   DOI
16 Daidzin inhibits mitochodrial aldehyde dehydrogenase and suppresses ethanol intake of Syrian golden hamsters. /
[ Keung, W.M.;Klyosov, A.A.;Vallee, B.L. ] / Proc Natl Acad Sci USA   DOI
17 Neurogenesis in the adult human hippocampus. /
[ Eriksson, P.S.;Perfilieva, E.;Björk-Eriksson, T.;Alborn, A. M.;Nordborg, C.;Peterson, D.A.;Gage, F.H. ] / Nat Med   DOI
18 Acupuncture increases cell proliferation in dentate gyrus after transient global in gerbis. /
[ Kim, E.-H.;Kim, Y.-J.;Lee, H.-J.;Huh, Y.;Chung, J.-H.;Seo, J.-C.;Kang, J.-E.;Lee, H.-J.;Yim, S.-V.;Kim, C.-J. ] / Neurosci Lett   DOI
19 Hippocampal nitric oxide synthase in the fetal guinea pig: effects of chronic prenatal ethanol exposure. /
[ Kimura, K.A.;Brien, J.F. ] / Dev Brain Res   DOI